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Embedding Retina Samples to Study Photoreceptor-Synapses Using Volume Electron Microscopy

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01:41 min

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May 24th, 2024

DOI :

10.3791/202328-v

May 24th, 2024

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Wenna Zhao*¹^,², Qingwen Yang*¹^,², Xiaoqian Lai¹^,², Jun Zhang¹^,²

¹State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, ²Laboratory of Retinal Physiology and Disease, School of Ophthalmology and Optometry, Wenzhou Medical University

* These authors contributed equally

Transkript

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Photoreceptor synapses

Volume Electron Microscopy

Sodium Cacodylate Buffer

Potassium Ferrocyanide

Osmium Tetroxide

Thio carbo hydrazide

Ethanol Concentration

Resin Infiltration

Focused Ion Beam Scanning Electron Microscopy

Cell Membrane Structure

Seriden

Visualizing Retinal Synaptic Structures in 3D with Volume Electron Microscopy

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