Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

1:28

Annealing Oligonucleotides (Applies Only to Freeze-dried DNA)

2:43

Golden Gate Assembly (Cost Efficient Method Using a Standard Mix)

4:19

Transformation and Colony Selection

5:19

Verification and Quantification of Terminator

7:55

Quantification of Six Different Terminators in pGR-Blue

9:02

Conclusion