Human Articular Cartilage Tissue Collection and Chondrocyte Culture
3:33
Genomic DNA Extraction
5:47
Dot Blot nalysis
7:55
Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
8:45
Conclusion
Transkript
The overall goal of this experiment is to determine the 5-Methylcytosine levels during chondrocyte dedifferentiation. This method shows can help answer key questions in cell-based cartilage defect treatment such as autologous chondrocyte implantat
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.