Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

1:04

3T3‐L1 Cell Culture and Maintenance

1:49

Starvation of 3T3‐L1 Cells

2:22

Preparation of FD‐Glucose Solutions with Different Concentrations

4:19

Treating Starved 3T3‐L1 Cells

5:03

Extracellular and Intercellular Measurements for Stimulated 3T3‐L1 Cells

6:00

Protein‐Based Normalization of Intercellular Glucose Uptake

6:35

Ex Vivo Measurement of Extracellular FD‐Glucose Depletion in Organs

7:59

Results: Extracellular FD‐Glucose Correlation with Intracellular Uptake and Kinetics of Extracellular FD‐Glucose Depletion

9:39

Conclusion