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Abstract

Medicine

Bilateral Renal Ischemia-Reperfusion Model for Acute Kidney Injury in Mice

Published: February 2nd, 2024

DOI:

10.3791/65838

1Kidney Disease Center, the First Affiliated Hospital, Zhejiang University School of Medicine, 2International Institute of Medicine, The Fourth Affiliated Hospital, Zhejiang University School of Medicine

* These authors contributed equally

Abstract

Acute kidney injury (AKI) is defined as a rapid decline in renal function, in which persistent kidney dysfunction gradually progresses to chronic kidney disease (CKD) due to the irreversible loss of nephrons and their maladaptive repair. In recent years, the incidence of AKI has been increasing concerning diverse etiologies, including volume depletion, sepsis, nephrotoxicity, muscle injury, and major trauma, in which ischemia-reperfusion injury (IRI) accounts for most episodes. Development of the IRI model in mice is induced by surgical clamping of the renal pedicles, which provides powerful and controllable tools for preclinical models of AKI. Importantly, the IRI model is deployed at different stages of the AKI development, especially in the processes of AKI to CKD. Despite the IRI model being widely practiced in many laboratories, a series of variables still influence the results of this model. Here, we describe the procedure of IRI model development to provide a repeatable and reliable method for researchers to explore the underlying pathogenesis in the development of AKI and the progression of AKI to CKD.

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Keywords Acute Kidney Injury

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