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Abstract

Medicine

Mouse Wound Models and Preparation of Single-Cell Suspensions

Published: September 27th, 2024

DOI:

10.3791/66499

1Medical School of Chinese People's Liberation Army, 2Department of Dermatology, Southwest Hospital, Army Medical University, 3Biomedical Treatment Center, the Seventh Medical Center, Chinese PLA General Hospital, 4The 83th Affiliated Hospital, Xinxiang Medical University, 5Department of Dermatology, the Seventh Medical Center, Chinese PLA General Hospital

Abstract

The management of acute full-thickness skin injuries presents a considerable challenge in clinical practice. The complexity of wound healing, involving diverse cell populations and the intricate wound microenvironment, complicates the assessment of treatment effects and their interactions using traditional experimental approaches. Single-cell transcriptome sequencing technology has revolutionized the understanding of cellular functions and mechanisms during wound healing. However, the variability in methodologies for creating mouse wound models introduces confounding factors that can impact experimental results. Furthermore, the process of dissociating mouse skin tissues presents significant technical hurdles, highlighting the critical need for high-quality single-cell suspensions for accurate transcriptome sequencing. Therefore, this study aims to optimize the construction of mouse wound models to accurately assess changes in wound closure while eliminating variables that could influence the subsequent sequencing result. Additionally, the preparation of single-cell suspensions was performed using both enzyme preparation protocols and test kit protocols to optimize cost and effectiveness.

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