Working with Centrifuges

Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA

Earth's gravitational force is capable of separating heterogeneous mixtures. However, many systems avert this type of separation owing to the length of time usually required in such cases. Centrifugation is one of the most powerful tools for the separation of heterogeneous mixtures¹. It involves the application of centripetal force for sedimentation of the heavier phase, leading to separation of the two phases, and is a commonly used technique in industrial and laboratory settings.

In a typical centrifugation process, particles suspended in a fluid are spun about the centrifuge axis of rotation, causing a force away from the axis. This force drives the particles to the bottom of the centrifuge sample holder at a rate which is dependent on the size and density of the particles. Centrifugation is therefore a technique to speed up the process of sedimentation.

To ensure safety in the usage of a centrifuge, the user should be properly trained and made aware of the hazards that might result from its usage. The following sections present a discussion of handling and safety while working with centrifuges.

1. General Protocol for Safe Centrifuge Usage

The laboratory supervisor or laboratory safety officer should ensure all users are properly trained in the selection of the rotor, sample tube, and proper operation of the centrifuge.
Any new user should be closely supervised by a trained user until he/she is confident on how to properly use the instrument.
Prior to using the instrument, each user should carefully read and understand the safety instructions for the specific centrifuge.

With the increased usage of centrifuges in laboratories and industry, the risk of hazards for using centrifuges has also increased. However, careful handling of the apparatus and proper knowledge of its use and safety measures can substantially mitigate accidents. A basic guideline for working with centrifuges is provided here. Nonetheless, procedures may vary marginally depending on the particular centrifuge machine used. Hence, it is always recommended to carefully read and understand the user manual of the particular

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