Proper Use of Autoclaves

Overview

Robert M. Rioux & Zhifeng Chen, Pennsylvania State University, University Park, PA

Autoclaving is one of the most commonly used methods in the laboratory for the purpose of decontamination. The most common items decontaminated with an autoclave are those that contact biological samples (typically those containing microorganisms). An autoclave works by utilizing pressurized high temperature steam to kill microorganisms present in the loaded materials. Sufficient steam flow and heat transfer are essential for highly efficient autoclaving, which are the key principles to consider when packaging materials. Safety needs to be considered when working with an autoclave due to the high pressure and temperature employed therein, which also sets limits on which materials are compatible and may necessitate special attention when packaging, loading, and unloading materials.

Procedure

1. Wear Proper Personal Protective Equipment (PPE)

The high pressure and temperature operating conditions encountered during autoclaving pose potential heat burn or explosion risks. Appropriate PPE includes a laboratory coat, safety glasses, heat resistant gloves, and closed-toe shoes; these must be worn when loading and unloading materials from the autoclave. When dealing with liquid samples, wear an apron and face shield as additional PPE.

2. Preparation of Materials

Application and Summary

Compared to chemical or radiation decontamination methods, autoclaving represents the most economic and practical method in the laboratory. However, to ensure safety (avoiding melting, heat burns, or explosion), identify autoclave-compatible materials and practice proper packaging, loading, and unloading. Ensuring sufficient decontamination efficiency requires container or bag materials and enough space between materials within the autoclave. Maintenance and inspection of the autoclave on a regular schedule are required

References

Autoclave Safety and Operation, 2015, Division of Research Safety, University of Illinois. at Autoclave Use, Environmental Health & Safety, Princeton University. atProper Use of a Steam Autoclave, 2016, Environmental Health & Safety, Arizona State University.
Fact Sheet: Using Autoclaves Safely, Environmental Health & Safety, California State University, Los Angeles.
Guideline for the Safe Use of Autoclaves, 2003, Environmental Health and Safety Service, University of Ottawa.