Name: high-resolution video tracking of locomotion in adult drosophila melanogaster
Uploaded: 2009-02-20T00:00:00
Description: Watch this Scientific Journal Video about High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster at JoVE.com

这项工作是由国家卫生部批准R01 GM54408授予立法会格里菲斯学院的支持。我们要感谢他对所有帮助我们的方腔设计和设置我们的实验中，弗兰克梅洛在布兰代斯大学的机加工车间建设我们的商会，丹瓦伦特和蒂姆Lebestky关于有助于分析问题的谈话弗雷德沃尔夫。

第1部分：苍蝇的饲养和管理<ol><li>苍蝇应该是生长在含有酵母的标准媒体瓶。您将需要大量的苍蝇，所以需要设立相应的十字架。苍蝇应在25℃生长在一个12小时的光：暗周期</li><li>苍蝇应收集羽化后不久（1-3天）。苍蝇可与使用二氧化碳扩散的阶段，并应包含蒸压或酵母媒体的测试管排序。我们使用雄性10只，存储到试管中。 </li><li>允许使用二氧化碳后运行行为实验前至少一天或两天。我们运?...

<ol>
	<li>Wolf, F. W., Rodan, A. R., Tsai, L. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-Resolution+Analysis+of+Ethanol-Induced+Locomotor+Stimulation+in+Drosophila.">High-Resolution Analysis of Ethanol-Induced Locomotor Stimulation in Drosophila.</a> J Neurosci. 22 (24), 11035-11044 (2002).</li><li>Soll, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+computers+in+understanding+how+animal+cells+crawl.">The use of computers in understanding how animal cells crawl.</a> International review of cytology. 163, 43-104 (1995).</li><li>Martin, J. R., Ollo, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+Drosophila+Ca2+/calmodulin-dependent+protein+kinase+(Caki)+is+localized+in+the+central+nervous+system+and+implicated+in+walking+speed.">A new Drosophila Ca2+/calmodulin-dependent protein kinase (Caki) is localized in the central nervous system and implicated in walking speed.</a> The EMBO journal. 15 (8), 1865-1876 (1996).</li><li>Levine, J. D., Funes, P., Dowse, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resetting+the+Circadian+Clock+by+Social+Experience+in+Drosophila+melanogaster.">Resetting the Circadian Clock by Social Experience in Drosophila melanogaster.</a> Science. 298, 2010-2012 (2002).</li></ol>

在我们的设置，一个中等强度的空气脉冲瞬时停止飞行运动。当空气脉冲结束时，苍蝇被释放这种静止状态和locomote通常，如在图2所示。因为这空气脉冲可有效地同步运动的人口，我们用它来开始审判，因此，我们也可以研究运动的发病势头打破刺激。然而，该试剂盒可以做没有空气脉冲自启动后的运动参数是由空气脉冲（图2）的影响。 一个经常发生的问题与运动自动分析?...

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<td>Sharp</td>
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<td>Tool</td>
<td>Cole Parmer</td>
<td>SY-32003-12</td>
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<td>DNASTAR, Inc.</td>
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high-resolution video tracking of locomotion in adult drosophila melanogaster

苍蝇提供了一个复杂的行为，由于在这一领域的研究人员提供的遗传工具的大量研究的重要模式。在学习的运动行为<em&gt;果蝇</em依赖的能力，能够在运动过程中或在一个给定的任务量化的变化。出于这个原因，一个高清晰度视频跟踪系统，如我们在本文中介绍的一个，是一个有价值的工具，在实时测量运动。我们的协议涉及的初始空气脉冲打破苍蝇的势头，在一个正方形室由30第二拍摄期间使用。一个跟踪程序是用来计算每个飞行的瞬时速度在10毫秒增量室。分析软件，然后编译这些数据，并输出各种参数，如平均速度，最大速度，在运动中所花费的时间，加速等，此协议将讨论行为的任务苍蝇正确的喂养和管理，处理无需麻醉或固定苍蝇，建立一个控制的环境中，和运行检测，从开始到结束。

Watch this Scientific Journal Video about High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster at JoVE.com

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

复杂的运动器官的行为在研究<em&gt;果蝇</em&gt;是依赖于量化在一个给定的飞行的运动变化的能力。本文演示了如何做到这一点使用了高分辨率的跟踪系统。

高分辨率视频跟踪移动的，在成年果蝇。

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

Research

JoVE Journal

Biology

Dissection of Larval CNS in Drosophila Melanogaster

在果蝇幼虫中枢神经系统的剖析

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

科研

生物学

High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae

高分辨率测量的气味驱动在果蝇幼虫的行为

Olfactory responses in Drosophila larvae have been traditionally studied in Petri dishes comprising a single peripheral odor source. In this behavioral ...

Visually Mediated Odor Tracking During Flight in Drosophila

在果蝇的飞行过程中可视介导的气味追踪

Flying insects use visual cues to stabilize their heading in a wind stream. Many animals additionally track odors carried in the wind. As such, visual ...

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

实时成像果蝇胚胎血细胞迁移

Many studies address cell migration using in vitro methods, whereas the physiologically relevant environment is that of the organism itself. Here we ...

Dissection of Oenocytes from Adult Drosophila melanogaster

Dissection of Oenocytes from Adult Drosophila melanogaster

从成人Oenocytes剖析果蝇</em

In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides ...

Isolation of Drosophila melanogaster Testes

Isolation of Drosophila melanogaster Testes

分离果蝇睾丸

The testes of Drosophila melanogaster provide an important model for the study of stem cell maintenance and differentiation, meiosis, and soma-germline ...

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

注射胰岛素和血淋巴的提取来衡量成人胰岛素敏感性果蝇</em

Conserved nutrient sensing mechanisms exist between mammal and fruit fly where peptides resembling mammalian insulin and glucagon, respectively function ...

Measurement of Lifespan in Drosophila melanogaster

Measurement of Lifespan in Drosophila melanogaster

寿命测量<em&gt;果蝇</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Maintenance of a Drosophila melanogaster Population Cage

Maintenance of a Drosophila melanogaster Population Cage

的维持<em&gt;果蝇</em&gt;人口笼

Large quantities of DNA, RNA, proteins and other cellular components are often required for biochemistry and molecular biology experiments. The short life ...

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

果蝇成人间接飞行肌的光学横截肌面积测定

Muscle mass wasting, known as muscle atrophy, is a common phenotype in Drosophila models of neuromuscular diseases. We have used the indirect flight ...