Name: drosophila larval nmj dissection
Uploaded: 2009-02-04T00:00:00
Description: Watch this Scientific Journal Video about Drosophila Larval NMJ Dissection at JoVE.com

在您开始之前<ol><li>所有的文化应该是生长在25 ° C </li><li> HL3.1清扫缓冲区可能提前做好准备。以50毫升的HL3.1等份，并保持它在冰上。 </li><li>准备在HL3.1 15毫升新鲜的3.5％的甲醛。保持它在冰上。 </li></ol>幼虫解剖<ol><li>放一个寒冷HL3.1的夹层板的下降。这将保持干燥和眩晕的动物，使其更容易与动物。 </li><li>用长镊子 ，选择一个流浪的第三龄幼虫和HL3.1下降。 </l...

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	<li>Jan, L. Y., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Properties+of+the+larval+neuromuscular+junction+in+Drosophila+Melanogaster.">Properties of the larval neuromuscular junction in Drosophila Melanogaster.</a> J. Physiol. 262, 189-214 (1976).</li><li>Johansen, J., Halpern, M. E., Johansen, K. M., Keshishian, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stereotypic+morphology+of+glutamatergic+synapses+on+identified+muscle+cells+of+Drosophila+larvae.">Stereotypic morphology of glutamatergic synapses on identified muscle cells of Drosophila larvae.</a> J Neurosci. 9, 710-725 (1989).</li><li>Kesheshian, H., Broadie, K., Chiba, A., Bate, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+neuromuscular+junction:+a+model+system+for+studying+synaptic+development+and+function.">The Drosophila neuromuscular junction: a model system for studying synaptic development and function.</a> Annu Rev Neurosci. 19, 545-575 (1996).</li><li>Vactor, D. V., Sink, H., Fambrough, D., Tsoo, R., Goodman, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genes+that+control+neuromuscular+specificity+in+Drosophila.">Genes that control neuromuscular specificity in Drosophila.</a> Cell. , 73-1137 (1993).</li><li>Feng, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+minimal+hemolymph-like+solution,+HL3.1,+for+physiological+recordings+at+the+neuromuscular+junctions+of+normal+and+mutant+Drosophila+larvae.">A modified minimal hemolymph-like solution, HL3.1, for physiological recordings at the neuromuscular junctions of normal and mutant Drosophila larvae.</a> J Neurogenet. 18 (2), 377-402 (2004).</li></ol>

在此视频中演示的剥离技术可用于准备各种实验技术的果蝇幼虫。如果荧光蛋白标记，幼虫可安装和成像立即。否则，免疫，可为了纪念特定的突触车厢。此外，电可以轻松地进行解剖幼虫，以评估神经递质的运作。 果蝇 NMJ年初以来，照亮它的基本结构和功能的研究已取得大受欢迎。1-4许多对果蝇NMJ的发展的研究中已确定的分子在脊椎?...

HL3夹层缓冲区：（毫米）70氯化钠，5氯化钾，0.2氯化钙，20氯化镁，10碳酸氢钠，5海藻糖，蔗糖，115 5 HEPES，pH值7.3。<br /<br /&gt; Sylgard夹层板：轻轻混匀（避免气泡）在烧杯10:1。倒入培养皿（任意大小）的混合物。允许SylGard治愈37C培养箱中过夜。

drosophila larval nmj dissection

“<em&gt;果蝇</em神经肌肉接头（NMJ）是一个建立的模型系统，用于研究突触发展和可塑性。广泛使用<em&gt;果蝇</em&gt;电机系统由于其高的无障碍。它可以分析单细胞的分辨率。有30％hemisegment肌肉的已知其安排在外围的体壁。在高保真的模式，共有35个运动神经元连接到这些肌肉。利用分子生物学和遗传学，可以建立转基因动物或突变体。然后，可以研究的NMJ的形态和功能上的发展后果。为了访问的研究NMJ，有必要仔细剖析每个幼虫。在这篇文章中，我们演示了如何正确地剖析<em&gt;果蝇</em&gt;幼虫NMJ的研究，通过消除所有的内部器官，而离开体壁不变。这种技术适用于准备成像，免疫组织化学，电或幼虫。

Watch this Scientific Journal Video about Drosophila Larval NMJ Dissection at JoVE.com

Drosophila Larval NMJ Dissection

此协议示范如何解剖<em&gt;果蝇</em&gt;幼虫免疫组织化学和/或神经肌肉接头处的成像的准备。

果蝇幼虫NMJ夹层

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

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Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

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We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Dissection of Oenocytes from Adult Drosophila melanogaster

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Dissection and Staining of Drosophila Larval Ovaries

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Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work ...

Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy

Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy

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Cell shape is critical for cell function. However, despite the importance of cell morphology, little is known about how individual cells generate specific ...