Name: murine renal transplantation procedure
Uploaded: 2009-07-10T00:00:00
Description: Watch this Scientific Journal Video about Murine Renal Transplantation Procedure at JoVE.com

<p class="jove_content">这些研究支持部分资金，由美国国立卫生研究院R01 AI36532 GAH。我们想确认在移植病理蒂博尔Nadasdy博士提供出色的支持，由已故的查尔斯博士欧罗斯发起这项研究工作提供了宝贵的指导和远见。 </p>

<p class="jove_title">捐赠者的器官丰收（2）： </p><ol><li>麻醉使用65mg/kg戊巴比妥IP捐助;异氟醚（2-3％）维持麻醉。 </li><li>一个从胸骨到耻骨正中切口进入捐助者的腹部，暴露左肾，大肠侧向移动到右侧。 </li><li>用8-0丝线缝合肾上腺和睾丸血管结扎和分裂隔离的左肾。 </li><li>调动的主动脉与肾动脉和静脉交界处以上。 </li><li>动员与肾动脉和静脉交界处的上方和下方的下腔静脉（IVC）。 </li><li>结扎肾动脉和静脉用8-0丝?...

<ol>
	<li>Russell, P. S., Chase, C. M., Colvin, R. B., Plate, J. M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Kidney+transplants+in+mice.+An+analysis+of+the+immune+status+of+mice+bearing+long-term,+H-2+incompatible+transplants.">Kidney transplants in mice. An analysis of the immune status of mice bearing long-term, H-2 incompatible transplants.</a> <em style='font-style: italic'>JExpMed</em>. <b>147</b>, 1449-1468 (1978).</li><li>Zhang, Z., Schlachta, C., Duff, J., Stiller, C., Grant, D., Zhong, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+techniques+for+kidney+transplantation+in+mice.">Improved techniques for kidney transplantation in mice.</a> <em style='font-style: italic'>Microsurgery</em>. <b>16</b> (2), 103-109 (1995).</li></ol>

<p class="jove_content">我们已经执行了约1200例肾移植技术的成功率&gt; 90％的小鼠。相比，心脏移植，肾移植的成功率通常比较低。然而，我们的实验室一直保持着较高的肾移植的成功率（&gt; 90％）。成功的关键点，以减少再灌注损伤供肾和受助人，并最大限度地减少出血和血栓形成的整个过程中和手术后。 （三）下列因素可能是最重要的是：（a）减少收件人出血，在手术过程中尽可能通过细心和轻柔的操作可能;（b...

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<td>Other</td>
<td>HESKA</td>
<td>CAT 5110-CREA</td>
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<td>Sulfatrim</td>
<td>Other</td>
<td>Actavis</td>
<td>R02109</td>
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murine renal transplantation procedure

<p class="jove_content">在小鼠肾原位移植是一个技术上具有挑战性的过程。 Russell等人虽然进行了第一次肾脏移植在小鼠体内超过30年的前（1）张等年后（2）精制而成，在世界上少数几个人已经掌握了此过程。在我们的实验室中，我们已成功地执行1200&gt; 90％的存活率原位肾移植。成功的关键点包括再灌注损伤，出血及血栓形成，无论是在程序和移植后，严格控制，并吻合，而不是使用的10-0 11-0缝合。 </p><p class="jove_content">手术后的护理和治疗的收件人，移植成功和评估是非常重要的。所有肾移植受者接受抗生素注射青霉素立即移植后，在饮水中不断sulfatrim。每天和全血中肌酐常规分析评估移植肾功能与便携机的I - STAT的整体动物健康评估。 </p>

Watch this Scientific Journal Video about Murine Renal Transplantation Procedure at JoVE.com

Murine Renal Transplantation Procedure

<p class="jove_content">在小鼠肾移植在技术上是一个具有挑战性的过程，需要仔细的术后护理和治疗的成功。</p>

小鼠肾移植程序

Renal orthotopic transplantation in mice is a technically challenging procedure. Although the first kidney transplants in mice were performed by Russell ...

Research

JoVE Journal

Biology

Heterotopic Heart Transplantation in Mice

小鼠异位心脏移植

The mouse heterotopic heart transplantation has been used widely since it was introduced by Drs. Corry and Russell in 1973. It is particularly valuable ...

科研

生物学

Small Bowel Transplantation In Mice

小鼠小肠移植

Since 1990, the development of tacrolimus-based immunosuppression and improved surgical techniques, the increased array of potent immunosuppressive ...

Murine Skin Transplantation

小鼠皮肤移植

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to ...

Generation of Human CD40-activated B cells

人CD40激活的B细胞的生成

CD40-activated B cells (CD40-B cells) have been identified as an alternative source of immuno-stimulatory antigen-presenting cells (APC) for cancer ...

Mammary Epithelial Transplant Procedure

乳腺上皮移植手术

This article describes and compares the fat pad clearance procedure developed by DeOme KB et al.1 and the sparing procedure developed by Brill B et al.2, ...

Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration

激光烧蚀斑马鱼Pronephros研究肾小管上皮再生

Acute kidney injury (AKI) is characterized by high mortality rates from deterioration of renal function over a period of hours or days that culminates in ...

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

在整个细胞群在单细胞水平上研究细胞周期蛋白B的蛋白水解

Equal distribution of chromosomes between the two daughter cells during cell division is a prerequisite for guaranteeing genetic stability 1. Inaccuracies ...

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

维甲酸的应用获取从初级小鼠成骨细胞骨细胞培养

The need for osteocyte cultures is well known to the community of bone researchers; isolation of primary osteocytes is difficult and produces low cell ...

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

在肾小管上皮细胞胞浆钙的测量流式细胞仪

A variety of cellular processes, both physiological and pathophysiological, require or are governed by calcium, including exocytosis, mitochondrial ...

Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart

为葡萄糖和脂肪酸氧化率在大鼠离体的测定方法

The mammalian heart is a major consumer of ATP and requires a constant supply of energy substrates for contraction. Not surprisingly, alterations of ...