July 2nd, 2009
•Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.
Related Videos
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Generation of Single-Cell Suspensions from Mouse Neural Tissue
Single-cell Suction Recordings from Mouse Cone Photoreceptors
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Generation of Mice Derived from Induced Pluripotent Stem Cells
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved