Name: isolation and transplantation of hematopoietic stem cells (hscs)
Uploaded: 2007-02-25T00:00:00
Description: Watch this Scientific Journal Video about Isolation and Transplantation of Hematopoietic Stem Cells HSCs at JoVE.com

Totaal beenmerg voorbereiding <ol><li> Vier B6 muizen worden opgeofferd en ontleed om dij-, scheen-, heup en de wervelkolom te verkrijgen. </li><li> Tijdens de dissecties, zijn de ledematen en de beenderen bewaard in PBS 2% FCS hitte geïnactiveerd (optioneel 2 mM EDA - dissectie medium). </li><li> De schone botten zijn gebroken met mortier en een stamper in dissectie medium. Een efficiënte manier om dit te doen is om dij, bovenbenen en heupen van elke muis, vervolgens 2 stekels breken op een moment. </li><li> De cel...

isolation and transplantation of hematopoietic stem cells (hscs)

Watch this Scientific Journal Video about Isolation and Transplantation of Hematopoietic Stem Cells HSCs at JoVE.com

Isolation and Transplantation of Hematopoietic Stem Cells HSCs

Isolatie en transplantatie van hematopoietische stamcellen (HSC)

Research

JoVE Journal

Biology

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Afleiding van hematopoietische stamcellen van muriene embryonale stamcellen

A stem cell is defined as a cell with the capacity to both self-renew and generate multiple differentiated progeny.  Embryonic stem cells (ESC) are ...

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Isolatie en analyse van hematopoietische stamcellen uit de placenta

Hematopoietic stem cells (HSCs) have the ability to self-renew and generate all cell types of the blood lineages throughout the lifetime of an individual. ...

Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

Isolatie van Vroege hematopoietische stamcellen van muizen dooierzak en AGM

In the mouse embryo, early hematopoiesis occurs simultaneously in multiple organs, which includes the yolk sac and aorta-gonad-mesonephros region. These ...

Generation of Human CD40-activated B cells

Generatie van de Mens CD40-geactiveerde B-cellen

CD40-activated B cells (CD40-B cells) have been identified as an alternative source of immuno-stimulatory antigen-presenting cells (APC) for cancer ...

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolatie en Afleiding van muis embryonale cellen Germinal

The ability of embryonic germinal cells (EG) to differentiate into primordial germinal cells (PGCs) and later into gametes during early developmental ...

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Lentivirale-gemedieerde Knockdown Tijdens het Ex Vivo Erytropoëse van humane hematopoietische stamcellen

Erythropoiesis is a commonly used model system to study cell differentiation. During erythropoiesis, pluripotent adult human hematopoietic stem cells ...

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Fenotypische analyse en isolatie van Murine hematopoëtische stamcellen en Lineage-geëngageerde voorouders

The bone marrow is the principal site where HSCs and more mature blood cells lineage progenitors reside and differentiate in an adult organism. HSCs ...

Induction and Analysis of Epithelial to Mesenchymal Transition

Inductie en analyse van epitheliale naar mesenchymale transitie

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Isolatie, Cultuur en Transplantatie van Muscle satelliet-cellen

Muscle satellite cells are a stem cell population required for postnatal skeletal muscle development and regeneration, accounting for 2-5% of sublaminal ...

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

Verbetering van de nauwkeurigheid van de flowcytometrische beoordeling van het mitochondriale membraan potentieel in hematopoietische stam en voorlopercellen door remming van effluxpompen

As cellular metabolism is a key regulator of hematopoietic stem cell (HSC) self-renewal, the various roles played by the mitochondria in hematopoietic ...