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23.1K Views
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20:38 min
February 25th, 2007
DOI :
10.3791/157-v
Chapters
0:00
Introduction
0:20
Isolating bone marrow cells
0:21
Dissecting mouse to isolate bones
1:03
Isolating tibia
1:40
Isolating femur
2:12
Isolating hip bone
2:50
Isolating spine
4:07
Cleaning bones
6:37
Crushing bones
9:33
Lineage depletion of bone marrow cells
14:06
Antibody staining for FACS
16:06
Transplantation into irradiated mouse
18:50
Discussion
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