Name: chick ex ovo culture and ex ovo cam assay: how it really works
Uploaded: 2009-11-30T14:45:00
Description: Watch this Scientific Journal Video about Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works at JoVE.com

저자는 아낌없이 종양 물질을 공급하는 우수한 기술 지원 및 교수 Ruebben에 대한 J. Kueper 및 J. Wittschier 감사하고 싶습니다.

모든 장비와 시약은 멸균 구입해야하거나 열 또는 증기 소독 또는 70 % ETOH와 소독을해야합니다. 동물 실험 기관에 의해 규정된 동물 실험 절차와 규정에 대한 동물의 보호 및 사용에 관한 NIH의 지침에 따라, 유럽 커뮤니티 이사회 지침 (86/609/EEC)에 따라 수행다고 저자 상태 관리 및 뒤스부르크 - 에센 대학 (독일)에서위원회 (IACUC)를 사용합니다. 1 부 : 달걀 ...

<ol>
	<li>Auerbach, R., Kubai, L., Knighton, D., Folkman, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+procedure+for+long-term+cultivation+of+chicken+embryos.">A simple procedure for long-term cultivation of chicken embryos.</a> Dev. Biol. 41, 391-394 (1974).</li><li>Tufan, A. C., Akdogan, I., Adiguzel, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Shell-less+culture+of+the+chick+embryo+as+a+model+system+in+the+study+of+development+neurobiology.">Shell-less culture of the chick embryo as a model system in the study of development neurobiology.</a> Neuroanatomy. 3, 8-11 (2004).</li><li>Deryugina, E. I., Quigley, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chick+embryo+chorioallantoic+membrane+models+to+quantify+angiogenesis+induced+by+inflammatory+and+tumor+cells+or+purified+effector+molecules.">Chick embryo chorioallantoic membrane models to quantify angiogenesis induced by inflammatory and tumor cells or purified effector molecules.</a> Methods Enzymology. 444, 21-44 (2008).</li><li>Ribatti, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chick+embryo+chorioallantoic+membrane+as+a+useful+tool+to+study+angiogenesis.">Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis.</a> Int. Rev. Cell Mol. Biol. 270, 181-224 (2008).</li><li>H&#246;ckel, M., Sasse, J., Wissler, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Purified+monocyte-derived+angiogenic+substance+(angiotropin)+stimulates+migration,+phenotypic+changes,+and+"tube+formation"+but+not+proliferation+of+capillary+endothelial+cells+in+vitro.">Purified monocyte-derived angiogenic substance (angiotropin) stimulates migration, phenotypic changes, and "tube formation" but not proliferation of capillary endothelial cells in vitro.</a> J. Cell Physiol. 133, 1-13 (1987).</li><li>Wissler, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+of+blood+vessel+patterns+by+Angio+Morphogens+[Angiotropins].">Engineering of blood vessel patterns by Angio Morphogens [Angiotropins].</a> Materialwiss. Werkstofftech. 32, 984-1008 (2001).</li><li>Kistler, H. B. . J. r. .., LaVail, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Penetration+of+horseradish+peroxidase+into+the+optic+nerve+after+vitreal+or+vascular+injections+in+the+developing+chick.">Penetration of horseradish peroxidase into the optic nerve after vitreal or vascular injections in the developing chick.</a> Invest Ophthalmol. Vis. Sci. 20, 705-716 (1981).</li><li>Halfter, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Change+in+embryonic+eye+size+and+retinal+cell+proliferation+following+intravitreal+injection+of+glycosaminoglycans.">Change in embryonic eye size and retinal cell proliferation following intravitreal injection of glycosaminoglycans.</a> Invest. Ophthalmol. Vis. Sci. 49, 3289-3298 (2008).</li><li>Oppitz, M., Busch, C., Shriek, G., Metzger, M., Just, L., Drews, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-malignant+migration+of+B16+mouse+melanoma+cells+in+the+neural+crest+and+invasive+growth+in+the+eye+cup+of+the+chick+embryo.">Non-malignant migration of B16 mouse melanoma cells in the neural crest and invasive growth in the eye cup of the chick embryo.</a> Melanoma Res. 17, 17-30 (2007).</li><li>Uchibayashi, T., Egawa, M., Nakajima, K., Hisazumi, H., Tanaka, M., Endo, Y., Sasaki, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responses+of+tumour+cell+lines+implanted+onto+the+chorioallantoic+membrane+of+chick+embryo+to+anticancer+agents+in+combination+with+hyperthermia.">Responses of tumour cell lines implanted onto the chorioallantoic membrane of chick embryo to anticancer agents in combination with hyperthermia.</a> Urol. Res. 20, 237-239 (1992).</li><li>Demir, R., Naschberger, L., Demir, I., Melling, N., Dimmler, A., Papadopoulus, T., Sturzl, M., Klein, P., Hohenberger, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hypoxia+generates+a+more+invasive+phenotype+of+tumour+cells:+an+in+vivo+experimental+setup+based+on+the+chorioallantoic+membrane.">Hypoxia generates a more invasive phenotype of tumour cells: an in vivo experimental setup based on the chorioallantoic membrane.</a> Pathol. Oncol. Res. , (2008).</li><li>Kunzi-Rapp, K., Genze, F., Kufer, R., Reich, E., Hautmann, R. E., Gschwend, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chorioallantoic+membrane+assay:+vascularized+3-dimensional+cell+culture+system+for+human+prostate+cancer+cells+as+an+animal+substitute+model.">Chorioallantoic membrane assay: vascularized 3-dimensional cell culture system for human prostate cancer cells as an animal substitute model.</a> J. Urol. 166, 1502-1507 (2001).</li><li>Hall, B. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Grafting+of+organs+and+tissues+to+the+chorioallantoic+membrane+of+the+embryonic+chick.">Grafting of organs and tissues to the chorioallantoic membrane of the embryonic chick.</a> TCA Manual. 4 (3), 881-883 (1978).</li><li>Kunzi-Rapp, K., Kaskel, P., Steiner, R., Peter, R. U., Krahn, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increased+blood+levels+of+human+S100+in+melanoma+chick+embryo+xenografts'+circulation.">Increased blood levels of human S100 in melanoma chick embryo xenografts' circulation.</a> Pigment Cell Res. 14, 9-13 (2001).</li><li>McFall, R. C., Sery, T. W., Makadon, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+a+new+continuous+cell+line+derived+from+a+human+retinoblastoma.">Characterization of a new continuous cell line derived from a human retinoblastoma.</a> Cancer Research. 37, 1003-1010 (1977).</li><li>Deryugina, E. I., Zijlstra, A., Partridge, J. J., Kupriyanova, T. A., Madsen, M. A., Papagiannakopoulos, T., Quigley, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unexpected+effect+of+matrix+metalloproteinase+down-regulation+on+vascular+intravasation+and+metastasis+of+human+fibrosarcoma+cells+selected+in+vivo+for+high+rates+of+dissemination.">Unexpected effect of matrix metalloproteinase down-regulation on vascular intravasation and metastasis of human fibrosarcoma cells selected in vivo for high rates of dissemination.</a> Cancer Research. 65, 10959-10969 (2005).</li><li>Kim, J., Kovalski, K., Ossowski, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=requirement+for+specific+proteases+in+cancer+cell+intravasation+as+revealed+by+a+novel+semiquantitative+PCR-based+assay.">requirement for specific proteases in cancer cell intravasation as revealed by a novel semiquantitative PCR-based assay.</a> Cell. 94, 353-362 (1998).</li></ol>

혁신 아니면 그냥 다른 아가씨 문화 프로토콜? 전 쉘 적은 문화 프로토콜과 함께 여자는 태아 1-4 전체 생존 요금은, 예를 들어 CA 낮은되었습니다. 30% 1. 이 비디오 문서에 설명되어있는 세련된 전부 비켜 문화 프로토콜은, 대조적으로, 50 % 이상 생존 속도를 허용합니다. 비켜 문화의 고전에 비해 여자는 배아의 전 비켜 문화 크게 CAM과 여자?...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Fertilized Eggs</td>
<td>Animal</td>
<td>S&ouml;ries Trockels, Germany</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Mice</td>
<td>Animal</td>
<td>Charles Rivers Laboratories</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Incubator Type 3000 with rotating egg tray</td>
<td>Tools</td>
<td>Siepmann GmbH Germany</td>
<td>9503</td>
<td>Maintain at 37&deg;C with relative humidity set above 60%</td>
</tr>
<tr>
<td>Egg incubator BSS 160</td>
<td>Tools</td>
<td>Grumbach, Germany</td>
<td>8101</td>
<td>Maintain at 37-387deg;C with relative humidity set above 60%</td>
</tr>
<tr>
<td>PBS</td>
<td>Reagent</td>
<td>Sigma</td>
<td>&nbsp;</td>
<td>PBS should be cold (&gt; 4&deg;C) and sterile
</td>
</tr>
<tr>
<td>Dulbecco's modified eagle's medium</td>
<td>Reagent</td>
<td>Sigma</td>
<td>D 6429</td>
<td>DMEM culture medium</td>
</tr>
<tr>
<td>Leibovitz's L-15 Medium</td>
<td>Reagent</td>
<td>Invitrogen</td>
<td>31415-029</td>
<td>here: collection medium for tumor samples</td>
</tr>
<tr>
<td>Tumor cell -specific medium</td>
<td>Reagent</td>
<td>Sigma</td>
<td>&nbsp;</td>
<td>each research group should use the medium they culture their tumors cells in or common culture media, e.g. Leibovitz's Medium or Dulbecco's modified eagle's medium supplemented with 15% fetal bovine serum (FBS), 4mM L-glutamine and 50 &mu;M insulin</td>
</tr>
<tr>
<td>70% EtOH</td>
<td>Reagent</td>
<td>Sigma</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Magnetic stir bar, triangled 80 mm</td>
<td>Tool</td>
<td>VWR</td>
<td>442-0391</td>
<td>A triangled magnetic stir bar serves well to crack the eggs shell.</td>
</tr>
<tr>
<td>Forceps DUMONT #5 </td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>11252-30</td>
<td>bevelled very fine shanks (0.05 mm x 0.02 mm tip)</td>
</tr>
<tr>
<td>Forceps DUMONT #7</td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>11271-30</td>
<td>curved shanks (0.07 mm x 0.10 mm tip)</td>
</tr>
<tr>
<td>Spring scissors, straight, 8cm</td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>15000-00</td>
<td>fine, small straight blades</td>
</tr>
<tr>
<td>Fine Iris scissors, straight</td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>14094-11</td>
<td>Use to cut our the CAM</td>
</tr>
<tr>
<td>Standard scissors, straight, sharp/blunt</td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>14007-14</td>
<td>Use for decapitation or cervical dislocation</td>
</tr>
<tr>
<td>microliter syringe 1702 TLLX, 25 &mu;l</td>
<td>Tool</td>
<td>Hamilton</td>
<td>80222</td>
<td>Use for injection into the vitreous</td>
</tr>
<tr>
<td>Hamilton 33-G needle (15 mm)</td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>18073-15</td>
<td>Use for injection into the vitreous</td>
</tr>
<tr>
<td>Sterile scalpels</td>
<td>Tool</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Use for dissecting tumor samples</td>
</tr>
<tr>
<td>Small drain spoon</td>
<td>Tool</td>
<td>Geuder, Germany</td>
<td>15758</td>
<td>Use to transfer of small chick embryos</td>
</tr>
<tr>
<td>Wax board with fixing pins</td>
<td>Tool</td>
<td>Self made</td>
<td>&nbsp;</td>
<td>Use to fix of animals for dissection</td>
</tr>
<tr>
<td>Petri dishes 60 x 15 mm</td>
<td>Tool</td>
<td>Greiner</td>
<td>628102</td>
<td>&nbsp;</td>
<tr>
<td>Petri dishes 92 x 16 mm</td>
<td>Tool</td>
<td>Sarstedt</td>
<td>82.1472</td>
<td>&nbsp;</td>
<tr>
<td>Sterile Petri dish 100 x 20 mm</td>
<td>Tool</td>
<td>Greiner</td>
<td>664160</td>
<td>extra deep, with spacers for ventilation</td>
</tr>
<tr>
<td>Beaker</td>
<td>Tool</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>300-600 ml</td>
</tr>
<tr>
<td>FALCON tubes</td>
<td>Tool</td>
<td>FALCON</td>
<td>&nbsp;</td>
<td>15 ml and 50 ml</td>
</tr>
<tr>
<td>Eppendorf tubes</td>
<td>Tool</td>
<td>Eppendorf</td>
<td>&nbsp;</td>
<td>1.5 ml and 2 ml</td>
</tr>
<tr>
<td>1ml pipette tips</td>
<td>Tool</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Use to cut plastic rings for application of substances / cells</td>
</tr>
<tr>
<td>Peripheral venous catheter (PVL)</td>
<td>Tool</td>
<td>Viggo&reg;</td>
<td>&nbsp;</td>
<td>Use to cut silicon rings from the tube for application of substances / cells</td>
</tr>
<tr>
<td>Pipettes and tips</td>
<td>Tool</td>
<td>Eppendorf / Abimed</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Autoclaved folded Filters 595 1/2, 110 mm diameter</td>
<td>Tool</td>
<td>Schleicher &amp; Schuell</td>
<td>311643</td>
<td>Carrier, which caused least irritation of the CAM; used in the paper</td>
</tr>
<tr>
<td>PTFE-Pledget, non resorbing, 6,3 x 152.4 x 1.6 mm</td>
<td>Tool</td>
<td>santec-medical</td>
<td>REF 277, LOT 832/511-1</td>
<td>Carrier; caused false positive results</td>
</tr>
<tr>
<td>Hxdroxyethylcellulose Tylose H 100000</td>
<td>Reagent</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Carrier mixed with Kollidon 17 PF; caused false positive results</td>
</tr>
<tr>
<td>Kollidon 17 PF</td>
<td>Reagent</td>
<td>BASF</td>
<td>S30200</td>
<td>mixed with Hydroxyethylcellulose; caused false positive results</td>
</tr>
<tr>
<td>Collagen Biomatrix TissuDura</td>
<td>Tool</td>
<td>Baxter Healthcare S.A.</td>
<td>REF B2246000999999</td>
<td>Carrier; caused false positive results</td>
</tr>
<tr>
<td>Round glass cover slips, 11 mm</td>
<td>Tool</td>
<td>Assistent Germany</td>
<td>1001/0011</td>
<td>Carrier; causes false positive results</td>
</tr>
<tr>
<td>Bovine Collagen Sponge</td>
<td>Tool</td>
<td>Wyeth</td>
<td>&nbsp;</td>
<td>Carrier; caused false positive results</td>
</tr>
<tr>
<td>Resodont Absorbable Collagen Membrane</td>
<td>Tool</td>
<td>Resorba Woundcare Germany</td>
<td>LOT 280303</td>
<td>Carrier; caused false positive results</td>
</tr>
<tr>
<td>Non-Woven Swabs</td>
<td>Tool</td>
<td>Fink &amp; Walter GmbH</td>
<td>REF 328132</td>
<td>Carrier; caused false positive results</td>
</tr>		</tbody>
		</table>
		</div>

chick ex ovo culture and ex ovo cam assay: how it really works

번식의 초기 단계에 치킨 계란은 체외에서 생체내 시스템의 사이에 있으며, angiogenesis를 공부뿐만 아니라 tumorigenesis를 공부뿐만 아니라 혈관 테스트 환경을 제공합니다. 여자 chorioallantoic 막 (CAM)가 개발되면, 그 혈관 네트워크는 쉽게 액세스할 수 조작하고 관찰하고 따라서 angiogenesis의 assays에 대한 최적의 설정을 제공합니다. 림프 시스템이 완전히 부화의 늦은 단계까지 개발이 아니므로, 여자의 난자는 종 특정 제한없이 이식할 조직 및 세포를 유지 수있는 자연 immunodeficient 호스트로 제공하고 있습니다. 개발 육성 이외에 allo을하고 xenografts, CAM의 혈관 네트워크는 종양 세포 intravasation, 보급, 그리고 혈관 체포와 체포 세포 마이크로 전이성 foci를 형성 extravasate 저장소를위한 고유 지원 환경을 제공합니다. 실험 목적을 위해, 최근 연구의 대부분의 CAM은 알 껍질을 통해 창문을 절단하고 실험 관찰과 효과의 사진을 문서에 대한 CAM과 가능성의 접근에 상당한 제한의 결과로, 비켜에서 실시되었다하여 노출되었습니다. 전혀 게시된 경우 여자는 배아의 쉘 적은 문화가 1-4 사용했을 때, 어떤 실험 자세한 내용은, 제공하지 않으며 있었다 이러한 문화의 생존 요금이 낮은되었습니다. 우리는 계란 내용의 이성적으로 제어 압출을 도입하여 크게 여자는 배아의 전 비켜 문화의 방법을 정제. 이 전 비켜 문화는 효과의 생체내 문서에 쉽게 활성화 및 배아의 실험 조작을 촉진, CAM 및 여자 태아의 접근을 향상시킵니다. 이것은 과학적인 질문이 다수를 성공적으로 응용 프로그램을 수행할 수 있습니다 (1) 개선 angiogenesis 분석 5,6로, (2) 실험 (3), 여자는 배아 아이 7-9의 유리체에서 촉진 주사에 대한 설정 의 보급과 intravasation위한 테스트 환경 (5 닭고기와 마우스 13은 잘 사지 꽃봉오리의 성공적인 이식과 문화에 대한 개선 유지 시스템으로 CAM 10-12에 주사를 설립 세포 라인에서 종양 세포 (4) 분산으로 )에 대한, 접목 전파 및 CAM 14 표면에 biopsies에서 얻은 탄탄한 기본 종양 조직의 다시 접목. 이 비디오를 문서에서 우리는 세련된 여자는 전 비켜 문화와 50 % 이상 생존 속도 CAM 분석의 설립을 설명합니다. 게다가 우리는 과학 응용 프로그램의 많은에 대한 예 비켜 문화의 성공적인 응용 프로그램의 단계 시범에 의해 단계를 제공합니다. 다니엘 S. 도일, 수잔느 D. 파사, 그리고 세바스찬 Gustmann이 연구에 동등하게 공헌했습니다.

Watch this Scientific Journal Video about Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works at JoVE.com

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

병아리 chorioallantoic 막 (CAM)은 angiogenesis와 tumorigenesis을 연구하는 독특한 자연 immunodeficient 지원 문화 환경입니다. 이 비디오 기사는 여자의 서로 다른 단계를 보여줍니다<em&gt; 예 비켜</em&gt; 문화, 가능성 angiogenic 물질 및 CAM의 표면에 종양 세포와 조직을 성공적으로 접종의 응용 프로그램입니다.

병아리 예 비켜 문화와 예 비켜 CAM 어세이 : 그게 정말로 작동하는 방법

Chicken eggs in the early phase of breeding are between in vitro and in vivo systems and provide a vascular test environment not only to study ...

Research

JoVE Journal

Biology

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

In Ovo Electroporations of HH Stage 10 Chicken Embryos

HH 스테이지 10 치킨 태아의 비켜 Electroporations에서

Large size and external development of the chicken embryo have long made it a valuable tool in the study of developmental biology.  With the advent of ...

연구

생물학

Method for Culture of Early Chick Embryos ex vivo (New Culture)

Method for Culture of Early Chick Embryos ex vivo New Culture

조기 칙 태아 예 생체내 (새로운 문화)의 문화에 대한 방법

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ...

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

활용 칙 신경 튜브에서 뉴런의 유전 정의된 그룹 Axonal 경로를 벗기기 비켜에서 Electroporation

Employment of enhancer elements to drive expression of reporter genes in neurons is a widely used paradigm for tracking axonal projection. For tracking ...

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

전 비켜</em이미징 및 미세 애플 리케이션에 적합> 치킨의 배아 문화 시스템

Understanding the relationships between genetic and microenvironmental factors that drive normal and malformed embryonic development is fundamental for ...

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

기본 인간 난관 상피 세포의 전의 VIVO 문화

Epithelial ovarian cancer is a leading cause of female cancer mortality in the United States. In contrast to other women-specific cancers, like breast and ...

In Ovo Electroporation in Embryonic Chick Retina

In Ovo Electroporation in Embryonic Chick Retina

배아 칙의 망막에서 비켜 Electroporation에

Chicken embryonic retina is an excellent tool to study retinal development in higher vertebrates. Because of large size and external development, it is ...

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

에 의한 이전 치킨 태아에 유전자 이체<em&gt; 전 비켜</em&gt; Electroporation

The chicken embryo provides an  excellent model system for studying gene function and regulation during  embryonic development. In ovo electroporation is ...

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart

소설<em&gt; 생체</em배아 마우스 사랑을&gt; 문화 방법

Developmental studies in the mouse are hampered  by the inaccessibility of the embryo during gestation. Thus, protocols to  isolate and culture individual ...

Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration

Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration

마우스 배아의 피부와 Melanoblast 이주의 라이브 영상의 생체 문화

Melanoblasts are the neural crest derived precursors of melanocytes; the cells responsible for producing the pigment in skin and hair. Melanoblasts ...

An Ex vivo Culture System to Study Thyroid Development

An Ex vivo Culture System to Study Thyroid Development

<em&gt; 예 생체 내</em&gt; 문화 시스템 갑상선 개발을 연구하는

The thyroid is a bilobated endocrine gland localized at the base of the neck, producing the thyroid hormones T3, T4, and calcitonin. T3 and T4 are ...