Name: lensless on-chip imaging of cells provides a new tool for high-throughput cell-biology and medical diagnostics
Uploaded: 2009-12-14T17:00:00
Description: Watch this Scientific Journal Video about Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics at JoVE.com

<html><p>在这里，我们讨论的是在卢卡斯[1-3]的实验程序。为了说明卢卡斯的概念证明，我们将描述为一个整体的血液样本的成像过程。 </p><p class="jove_title">答：成像设置</p><p class="jove_content">卢卡斯成像平台表现出显着的优势，对现有的护理点流式细胞仪和医疗诊断工具，尤其是对资源有限的环境，提供成本效益和紧凑的替代。检测细胞的形象，而不是卢卡斯，而不是捕捉的是从每个单元格的背景光的散射光的干扰?...

<ol>
	<li>Seo, S., Su, T., Tseng, D. K., Erlinger, A., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term="Lensfree+Holographic+Imaging+for+On-Chip+Cytometry+and+Diagnostics".">"Lensfree Holographic Imaging for On-Chip Cytometry and Diagnostics".</a> <em style='font-style: italic'>Lab Chip</em>. , (2009).</li><li>Su, T., Seo, S., Erlinger, A., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term="High-throughput+Imaging+and+Characterization+of+a+Heterogeneous+Cell+Solution+on+a+Chip"+.">"High-throughput Imaging and Characterization of a Heterogeneous Cell Solution on a Chip" .</a> <em style='font-style: italic'>Biotechnology and Bioengineering</em>. , (2008).</li><li>Su, T., Seo, S., Erlinger, A., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term="Multi-color+LUCAS:+Lensfree+on-chip+cytometry+using+tunable+monochromatic+illumination+and+digital+noise+reduction".">"Multi-color LUCAS: Lensfree on-chip cytometry using tunable monochromatic illumination and digital noise reduction".</a> <em style='font-style: italic'>Cellular and Molecular Bioengineering</em>. , (2008).</li></ol>

<p class="jove_content">我们已经说明，卢卡斯平台可以准确计数和基于全息签名的芯片识别各种micro-objects/cells，点的医疗诊断和高通量细胞生物学提供了一种有前途的工具。为了准确地处理所记录的全息图案，我们实现了一个自定义开发卢卡斯决定的软件。这种算法，它采用了统计培训卢卡斯图像所造成的衍射图像数据库，确定细胞内的异构解决方案的各种功能，单个细胞的类型和相对位置进行分类和应用阈值后，放...

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lensless on-chip imaging of cells provides a new tool for high-throughput cell-biology and medical diagnostics

<p class="jove_content">工作与其他镜头和光学组件的物镜使用常规的光学显微镜图像细胞。这种传统的方法虽然很有效，有一定的局限性，成像平台，使之与先进国家的最先进的微流体兼容的小型化。在这份报告中，我们引入一个无透镜片上的成像的概念称为卢卡斯的实验细节（<strong<em&gt; L</em</strong&gt; ensless<strong<em&gt; U</em</strong&gt; LTRA宽领域的视图<strong<em&gt; C</em</strong&gt; ELL监测<strong<em&gt; A</em</strong&gt; rray平台的基础上<strong<em&gt; S</em</strong&gt;哈多成像），不需要任何显微镜目标或其他笨重的光学元件的图像通过一个超宽的领域可以跨越大如一个异构的细胞解决方案〜18厘米<sup&gt; 2</sup&gt;。此外，不同于传统的显微镜，卢卡斯可以形象的利益异质细胞多〜5毫米的深度场的解决方案，而不需要重新聚焦对应〜9 mL样品量。这种成像平台记录的每一个细胞，其视野内的兴趣和自动数字处理这些细胞阴影的阴影（即，数字全息图无透镜），可确定的类型，数量和细胞内的解决方案的相对位置。因为它不需要任何笨重的光学元件或机械扫描阶段，它提供了一个显着小型化的平台，同时降低了成本，这一点非常重要的护理诊断工具，尤其是点。此外，这个平台的成像吞吐量订单数量级比传统的光学显微镜，高通量细胞生物学实验，这可能是极为宝贵的更好。</p>

Watch this Scientific Journal Video about Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics at JoVE.com

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

<p class="jove_content">Lensfree片上的成像和细胞的特性说明。这片上的细胞成像方法用于医疗诊断和高通量细胞生物学的应用提供了一个紧凑和成本效益的工具，使得它特别适合资源贫乏地区。</p>

无透镜片上的细胞成像提供了一种高通量细胞生物学和医疗诊断的新工具

Conventional optical microscopes image cells by use of objective lenses that work together with other lenses and optical components. While quite ...

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