Name: spinal cord electrophysiology
Uploaded: 2010-01-18T16:00:00
Description: Watch this Scientific Journal Video about Spinal Cord Electrophysiology at JoVE.com

शमूएल एल Pfaff जीन एक्सप्रेशन प्रयोगशालाओं में जैविक अध्ययन के लिए सॉल्क संस्थान में एक प्रोफेसर और हॉवर्ड ह्यूजेस मेडिकल इंस्टीट्यूट में एक अन्वेषक है. यह काम क्रिस्टोफर और दाना रीव फाउंडेशन द्वारा समर्थित किया गया था. जो Belcovson, केंट Schnoeker और सॉल्क संस्थान में मल्टीमीडिया संसाधन में माइक सुलिवान फोटोग्राफी और संपादन के साथ सहायता प्रदान की है.

1. कृत्रिम मस्तिष्कमेरु द्रव (aCSF) 1 तैयार करें. हम पहले aCSF की एक 10X शेयर मैग्नीशियम या कैल्शियम के बिना एक 2L तैयार. Millimolar में सूचीबद्ध अभिकर्मकों. सूचि संख्या / सिग्मा Aldrich देखें. <table border="1" cellpadding="0"><tbody><tr><td colspan="4"> 2 लीटर ...

<ol>
	<li>Myers, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cholinergic+input+is+required+during+embryonic+development+to+mediate+proper+assembly+of+spinal+locomotor+circuits.">Cholinergic input is required during embryonic development to mediate proper assembly of spinal locomotor circuits.</a> Neuron. 46, 37-49 (2005).</li><li>Goulding, M., Pfaff, S. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+circuits+that+generate+simple+rhythmic+behaviors+in+vertebrates.">Development of circuits that generate simple rhythmic behaviors in vertebrates.</a> Curr Opin Neurobiol. 15 (1), 14-20 (2005).</li><li>Gallarda, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Segregation+of+axial+motor+and+sensory+pathways+via+heterotypic+trans-axonal+signaling.">Segregation of axial motor and sensory pathways via heterotypic trans-axonal signaling.</a> Science. 320, (2008).</li><li>Gosgnach, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=V1+spinal+neurons+regulate+the+speed+of+vertebrate+locomotor+outputs.">V1 spinal neurons regulate the speed of vertebrate locomotor outputs.</a> Nature. 440 (7081), 215-219 (2006).</li><li>Lanuza, G. M., Gosgnach, S., Pierani, A., Jessell, T. M., Goulding, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+identification+of+spinal+interneurons+that+coordinate+left-right+locomotor+activity+necessary+for+walking+movements.">Genetic identification of spinal interneurons that coordinate left-right locomotor activity necessary for walking movements.</a> Neuron. 42 (3), 375-386 (2004).</li><li>Jiang, Z., Carlin, K. P., Brownstone, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+in+vitro+functionally+mature+mouse+spinal+cord+preparation+for+the+study+of+spinal+motor+networks.">An in vitro functionally mature mouse spinal cord preparation for the study of spinal motor networks.</a> Brain Res. 816 (2), 493-499 (1999).</li><li>Ziskind-Conhaim, L., Gao, B. X., Hinckley, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ethanol+dual+modulatory+actions+on+spontaneous+postsynaptic+currents+in+spinal+motoneurons.">Ethanol dual modulatory actions on spontaneous postsynaptic currents in spinal motoneurons.</a> J Neurophysiol. 89 (2), 806-813 (2003).</li><li>Tabak, J., Rinzel, J., O'Donovan, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+activity-dependent+network+depression+in+the+expression+and+self-regulation+of+spontaneous+activity+in+the+developing+spinal+cord.">The role of activity-dependent network depression in the expression and self-regulation of spontaneous activity in the developing spinal cord.</a> J Neurosci. 21 (22), 8966-8978 (2001).</li><li>Chub, N., Mentis, G. Z., O'Donovan, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chloride-sensitive+MEQ+fluorescence+in+chick+embryo+motoneurons+following+manipulations+of+chloride+and+during+spontaneous+network+activity.">Chloride-sensitive MEQ fluorescence in chick embryo motoneurons following manipulations of chloride and during spontaneous network activity.</a> J Neurophysiol. 95 (1), 323-330 (2006).</li></ol>

पृथक नवजात रीढ़ की हड्डी में तंत्रिका तंत्र development1, 2 अध्ययन का एक विनयशील तरीका प्रदान करता है. नवजात कृन्तकों की काठ का रीढ़ की हड्डी के भीतर, केंद्रीय पैटर्न पैदा circuitry के न्यूरोट्रांसमीटर की उपस्थिति म?...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>KCl</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>P-9333</td>
<td>&nbsp;</td>
<tr>
<td>NaCl	</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>S-7653</td>
<td>&nbsp;</td>
<tr>
<td>NaHCO3</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>S-6297</td>
<td>&nbsp;</td>
<tr>
<td>NaH2PO4</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>S-9638</td>
<td>&nbsp;</td>
<tr>
<td>Glucose</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>D-9434</td>
<td>&nbsp;</td>
<tr>
<td>CaCl2</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>C-5080</td>
<td>&nbsp;</td>
<tr>
<td>MgSO4</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>M-5921</td>
<td>&nbsp;</td>
<tr>
<td>Large Scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>14070-12</td>
<td>&nbsp;</td>
<tr>
<td>Forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11050-10</td>
<td>&nbsp;</td>
<tr>
<td>Fine Scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>15000-10</td>
<td>&nbsp;</td>
<tr>
<td>Insect pins</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>26002-10</td>
<td>&nbsp;</td>
<tr>
<td>Sylgard 184 (Dow-Corning)</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>1L volumetric flask</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>100mL volumetric flask</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

spinal cord electrophysiology

नवजात माउस रीढ़ की हड्डी में तंत्रिका circuitries और हरकत आंदोलन के विकास के अध्ययन के लिए एक मॉडल है. हम रीढ़ की हड्डी विच्छेदन और रिकॉर्डिंग स्नान कृत्रिम मस्तिष्कमेरु द्रव हरकत अध्ययन के लिए इस्तेमाल किया की तैयारी को प्रदर्शित करता है. एक बार dissected, रीढ़ की हड्डी वेंट्रल तंत्रिका जड़ों एक रिकॉर्डिंग इलेक्ट्रोड के लिए संलग्न किया जा काठ की हड्डी के भीतर केंद्रीय पैटर्न पैदा circuitry के electrophysiologic संकेतों को रिकॉर्ड कर सकते हैं.

Watch this Scientific Journal Video about Spinal Cord Electrophysiology at JoVE.com

Spinal Cord Electrophysiology

नवजात electrophysiologic अध्ययन के लिए माउस रीढ़ की हड्डी के अलगाव का एक प्रदर्शन.

स्पाइनल कॉर्ड इलैक्ट्रोफिजियोलॉजी

The neonatal mouse spinal cord is a model for studying the development of neural circuitries and locomotor movement. We demonstrate the spinal cord ...

Research

JoVE Journal

Biology

Applying Microfluidics to Electrophysiology

इलैक्ट्रोफिजियोलॉजी के लिए Microfluidics लागू

Microfluidics can be integrated with standard electrophysiology techniques to allow new experimental modalities.  Specifically, the motivation for the ...

Preparation of Artificial Bilayers for Electrophysiology Experiments

कृत्रिम bilayers इलैक्ट्रोफिजियोलॉजी प्रयोगों के लिए तैयार

Planar lipid bilayers, also called artificial lipid bilayers, allow you to study ion-conducting channels in a well-defined environment. These bilayers can ...

Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord

परिधीय तंत्रिका कलम बांधने का काम और मैट्रिक्स मॉडुलन मेल घायल चूहा स्पाइनल कॉर्ड की मरम्मत

Traumatic injury to the spinal cord (SCI) causes death of neurons, disruption of motor and sensory nerve fiber (axon) pathways and disruption of ...

Semi-automated Optical Heartbeat Analysis of Small Hearts

छोटे दिल की अर्ध स्वचालित ऑप्टिकल हार्टबीट विश्लेषण

We have developed a method for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts (Fink, et. al., 2009). Our ...

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Diffusible संकेतन अणुओं की योग्यता को भ्रूणीय स्पाइनल Commissural axons नई दिशा परख

Dorsal commissural axons in the vertebrate spinal cord1 have been an invaluable model system in which to identify axon guidance signals.  Here, we ...

Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography

धमनी चिकनी पेशी Kv7 पोटेशियम चैनल फंक्शन तलाश पैच दबाना इलैक्ट्रोफिजियोलॉजी और दबाव Myography का उपयोग

Contraction  or relaxation of smooth muscle cells within the walls of resistance arteries  determines the artery diameter and thereby controls flow of ...

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

इमेजिंग और पैच दबाना इलैक्ट्रोफिजियोलॉजी के लिए विशालकाय Liposome तैयारी

The reconstitution of ion channels into chemically defined lipid membranes for electrophysiological recording has been a powerful technique to identify ...

Introduction to Solid Supported Membrane Based Electrophysiology

इलैक्ट्रोफिजियोलॉजी आधार ठोस समर्थित झिल्ली का परिचय

The electrophysiological method we present is based on a solid supported membrane (SSM) composed of an octadecanethiol layer chemisorbed on a gold coated ...

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

चूहे में रैपिड अवेध्य काठ intrathecal इंजेक्शन के माध्यम से विवो siRNA अभिकर्मक और रीढ़ की हड्डी में जीन पछाड़ना में

This report describes a step-by-step guide to the technique of acute intrathecal needle injections in a noninvasive manner, i.e. independent of catheter ...

Cystometric and External Urethral Sphincter Measurements in Awake Rats with Implanted Catheter and Electrodes Allowing for Repeated Measurements

Cystometric और बाह्य मूत्रमार्ग प्रत्यारोपित कैथेटर और दोहराया माप के लिए अनुमति इलेक्ट्रोड के साथ जाग चूहों में दबानेवाला माप

Lower urinary tract function is mainly assessed by means of cystometric bladder function analysis in rodents. Conventional cystometries are usually ...