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07:06 min
January 14th, 2010
DOI :
10.3791/1704-v
Chapters
0:00
Title
0:30
Introduction
1:03
Preparing for Microinjection
3:14
Microinjection and Oocyte Culture
4:35
Fixation, Dehydration, and Storage of Oocytes
5:35
Imaging RNA Localization by Confocal Microscopy
5:59
RNA Localization in Xenopus Oocytes: Representative Results
6:28
Conclusion
Summary
Visualization of in vivo RNA transport is accomplished by microinjection of fluorescently labeled RNA transcripts into Xenopus oocytes, followed by confocal microscopy.
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