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09:28 min
April 19th, 2010
DOI :
10.3791/1779-v
Chapters
0:00
Title
0:31
Introduction
0:57
Replica Molding of PDMS from SU-8 Master
2:02
Bonding and Assembling the PDMS Device
3:40
Growing Highly Motile E.coli
4:49
Monitoring Chemotaxis
5:52
Data Analysis
7:51
Representative Chemotaxis Results
8:59
Conclusion
Summary
This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.
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