Name: multielectrode array recordings of the vomeronasal epithelium
Uploaded: 2010-03-01T16:25:00
Description: Watch this Scientific Journal Video about Multielectrode Array Recordings of the Vomeronasal Epithelium at JoVE.com

我们要感谢圣灵实验室成员。这项工作得到了国家耳聋与沟通障碍研究所（R01 DC005964）和美国国家科学基金会IGERT 0548890（HAA）。 对动物的实验是在按照美国的动物福利法案和美国国立卫生指引研究院，是由华盛顿大学的动物护理和使用委员会批准。

第1部分。电气记录仪器<ol><li>我们使用一个平面与10微米平面氮化钛，氮化硅绝缘电极的多电极阵列（ALA的科学仪器）。被安排在60个电极组成一个中心，中心间距30微米的5X6格的两个领域。 （其他配置也有可能与记录面直径在10微米的电极比孤立的单个神经元的活动方面有较大的电极。） </li><li>电信号放大（放大1000倍），使用MEA 1060放大器（ALA科学仪器）。信号采集采用数据采集卡（美国国家?...

<ol>
	<li>Dulac, C., Torelo, A. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+detection+of+pheromone+signals+in+mammals:+from+genes+to+behaviour.">Molecular detection of pheromone signals in mammals: from genes to behaviour.</a> Nat Rev Neurosci. 4, 551-562 (2003).</li><li>Holy, T. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responses+of+Vomeronasal+neurons+to+natural+stimuli.">Responses of Vomeronasal neurons to natural stimuli.</a> Science. 289, 1569-1572 (2000).</li><li>Nodari, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sulfated+steroids+as+natural+ligands+of+mouse+pheromone-sensing+neurons.">Sulfated steroids as natural ligands of mouse pheromone-sensing neurons.</a> J Neurosci. 28, 6407-6418 (2008).</li></ol>

多电极阵列记录，让我们一个人口众多的神经元的同步活动进行监控的能力。这是一个有用的工具，用于探测感官系统的性能，特别是VNO。与主要的嗅觉系统，VNO检测液相的刺激。此外，VNO是一种异质性的组织。有大约300个不同类型的鼠标 1受体可能与不同的反应概况， 。结合机械臂的多边环境协定的录音，让我们能够可靠地提供液体刺激的神经元和身份回应electrophysiologically庞大和多样?...

multielectrode array recordings of the vomeronasal epithelium

了解神经回路需要方法，同时从许多神经元记录。对于<em&gt;在体外</em&gt;研究之一，现有技术是平面的多电极阵列（MEA）记录。在这里，我们记录多边环境协定的使用鼠标犁鼻器（VNO）检测信息素和社会线索，通过感觉神经元表达数百种类型的受体的多样化的人口，它起着至关重要的作用的研究。结合MEA记录机器人的液体处理程序，以提供化学刺激的感官反应，大量的神经元和不同的人口可以被记录下来。准备允许我们从鼠标中删除的犁鼻器与电池的化学品或潜在配体的完整的神经上皮和刺激，同时监控几个小时的神经元的电活动。因此，这项技术作为一个有用的方法评估配体的活动，以及探索受体神经元的属性。我们目前需要准备的犁鼻器上皮，多边环境协定的记录，和化学刺激的技术。

Watch this Scientific Journal Video about Multielectrode Array Recordings of the Vomeronasal Epithelium at JoVE.com

Multielectrode Array Recordings of the Vomeronasal Epithelium

多电极阵列（MEA）的录音提供了一个人口众多的神经元电活动的研究方法。在这里，我们目前的一个多边环境协定的准备的细节，从小鼠犁鼻上皮的记录，同时刺激组织。

多电极阵列的犁鼻上皮录音

Understanding neural circuits requires methods to record from many neurons simultaneously. For in vitro studies, one currently available technology is ...

Research

JoVE Journal

Neuroscience

Single-unit In vivo Recordings from the Optic Chiasm of Rat

Single-unit In vivo Recordings from the Optic Chiasm of Rat

单机在体内</em从大鼠视交叉>录音

Information about the visual world is transmitted to the brain in sequences of action potentials in retinal ganglion cell axons that make up the optic ...

科研

神经科学

Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster

Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster

从巨纤维通路的电生理记录 D。果蝇</em

When startled adult D. melanogaster react by jumping into the air and flying away. In many invertebrate species, including D. melanogaster, the "escape" ...

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

器官型培养的神经元雪崩多电极阵列记录

The cortex is spontaneously active, even in the absence of any particular input or motor output.  During development, this activity is important for the ...

Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation

成像遥感信息素在小鼠犁鼻急性组织切片的制备

Peter Karlson and Martin L&uuml;scher used the term pheromone for the first time in 19591 to describe chemicals used for intra-species communication. ...

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor

在犁鼻器官的切片准备工作表示一个基因编码的钙传感器成像神经元的反应

The vomeronasal organ (VNO) detects chemosensory signals that carry information about the social, sexual and reproductive status of the individuals within ...

Ex Vivo Preparations of the Intact Vomeronasal Organ and Accessory Olfactory Bulb

Ex Vivo Preparations of the Intact Vomeronasal Organ and Accessory Olfactory Bulb

完整的犁鼻器和副嗅球的体外制剂

The mouse accessory olfactory system (AOS) is a specialized sensory pathway for detecting nonvolatile social odors, pheromones, and kairomones. The first ...

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

在小鼠犁鼻急性组织切片定义细胞群的深入生理学分析

In most mammals, the vomeronasal organ (VNO) is a chemosensory structure that detects both hetero- and conspecific social cues. Vomeronasal sensory ...

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

全细胞电压钳记录的电生理方法<em&gt;果蝇</em&gt;光感受器

Whole-cell voltage clamp recordings from Drosophila melanogaster photoreceptors have revolutionized the field of invertebrate visual transduction, ...

Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

从果蝇幼虫神经肌肉交界处的突触电流的焦 Macropatch 记录

Drosophila neuromuscular junction (NMJ) is an excellent model system to study glutamatergic synaptic transmission. We describe the technique of focal ...

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

微电极阵列技术在完整仔猪脑麻醉诱导神经毒性研究中的应用

Every year, millions of children undergo anesthesia for a multitude of procedures. However, studies in both animals and humans have called into question ...