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30.9K Views
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14:14 min
April 29th, 2007
DOI :
10.3791/186-v
Chapters
0:00
Estimation of bead concentrations
0:10
Introduction
0:46
Reagents
1:27
Coating beads with HUVEC cells
3:15
Washing beads
5:34
8:02
Forming the fibrin matrix
10:22
Layering lung fibroblasts atop the fibrin gel
Summary
This video demonstrates the protocol of an in vitro angiogenesis assay that recapitulates several stages of angiogenesis. Time-lapse images of sprouting, lumen formation, branching and anastomosis - key features of angiogenesis - are shown.
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