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DOI :
10.3791/1899-v
April 27th, 2010
Chapters
0:00
Title
0:48
Introduction
4:34
Immunoprecipitation (IP)
1:28
Pulse-chase Labeling and Protein Extraction
9:24
Conclusion
8:05
Analysis of N-glycans from NIH 3T3 Glycoproteins Representative Results
5:38
Deglycosylation and Preparing the Samples for HPLCE Analysis
我们描述了一种通过改变早期的生活后,其在哺乳动物细胞中合成的糖蛋白的N -连接聚糖分析方法。这是通过分析代谢标记聚糖,从糖蛋白和高效液相色谱法检查酶的释放脉冲追逐。
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