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DOI :
10.3791/1899-v
April 27th, 2010
Chapters
0:00
Title
0:48
Introduction
4:34
Immunoprecipitation (IP)
1:28
Pulse-chase Labeling and Protein Extraction
9:24
Conclusion
8:05
Analysis of N-glycans from NIH 3T3 Glycoproteins Representative Results
5:38
Deglycosylation and Preparing the Samples for HPLCE Analysis
我々は、哺乳類細胞におけるそれらの生合成後の糖タンパク質の初期の生活を通じて、N -結合型グリカンの変化の分析のための方法を説明します。これは、代謝的に標識した糖鎖、糖タンパク質およびHPLCによる検討からの酵素遊離のパルス - チェイス分析によって達成されます。
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