Name: proboscis extension response (per) assay in drosophila
Uploaded: 2007-04-29T00:00:00
Description: Watch this Scientific Journal Video about Proboscis Extension Response PER Assay in Drosophila at JoVE.com

<p>我们感谢保罗格拉汉姆在相机的视频演示男子。 </p>

<p class="jove_step">饥饿</p><ol><li>首先，饿死的苍蝇。 </li><li>放一个小块，用清水浸泡在一个空的小瓶金擦拭。 </li><li>转让20-30精心喂养苍蝇的空药瓶。 </li><li>检查每12 -24小时，以确保纸张湿润。 </li><li>饿死36-72小时的飞行。 </li></ol><p class="jove_step">修正飞</p><ol><li>采取一飞在抽吸。 </li><li>将200μl的黄色提示主持开幕。同时翻转和吹就可以了，你可以挤飞入黄尖。 </li><li>轻轻涂抹一些空中飞了起来推到一个无法动弹?...

<ol>
	<li>Dethier, V. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>The Hungry Fly</em>. , (1976).</li></ol>

<p>在此演示中，我们已经经历了长鼻扩展响应检测的所有步骤。该方法听起来很简单，但在许多其他行为检测，许多小东西可以防止的检测工作。我们都经历了许多需要认真注意这些步骤。在这里，我再次强调一些最重要的因素。 </p><p>这样的一个因素是芯子的形状。我已经展示了一些很好的例子，但当然，在现实中的一切将是一个非常好，非常糟糕的连续光谱。在开始的时候，你可能会使用非常好的一面的东西?...

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proboscis extension response (per) assay in drosophila

<p class="jove_content">长鼻延伸反应（PER）是一种味道已在果蝇以及用于蜜蜂的行为检测。 </p><p class="jove_content">在果蝇的口（唇瓣）表面有毛发状结构称为感受器，这房子的味道神经元。当一个有吸引力的物质接触到的唇瓣，飞扩展它的长鼻，消耗材料。长鼻扩展响应（PER）的检测措施，这种味道的行为反应，和对食物的喜好在一个单一的飞行学习，它是一个有用的方法。各种糖，如蔗糖，葡萄糖和果糖，解决方案是非常有吸引力的苍蝇。厌恶物质的影响，也可以作为每减少测试时，在甜美的解决方案混合。 </p><p class="jove_content">尽管简单的基本程序，有很多事情可以阻止它的工作。需要注意的因素之一，是动态的响应状态。所需的饥饿时间带来的飞行，以适当的响应状态迥然不同从36到72小时。我们建立了一系列的控制，以评估的飞行状态，并允许筛选出无响应或超反应的动物个体。另一个重要因素是影响的程度和接触的唇瓣，这将是难以用言语来形容的地位。此视频演示，演示所有这些一起，与其他一些改进，会增加这种方法的可重复性。 </p>

Watch this Scientific Journal Video about Proboscis Extension Response PER Assay in Drosophila at JoVE.com

Proboscis Extension Response PER Assay in Drosophila

<p class="jove_content">长鼻扩展响应或每一个味道已在果蝇以及用于蜜蜂的行为检测。长鼻当一个有吸引力的物质接触，飞扩展它的长鼻消耗物质。各种糖的解决方案非常有吸引力的苍蝇。</p>

长鼻扩展响应（PER）的含量在果蝇

Proboscis extension response (PER) is a taste behavior assay that has been used in flies as well as in honeybees.
On the surface of the fly's mouth ...

Research

JoVE Journal

Biology

Proboscis Extension Response (PER) Assay in Drosophila

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

引物延伸捕获：从严重退化的DNA来源有针对性的顺序检索

We present a method of targeted DNA sequence retrieval from DNA sources which are heavily degraded and contaminated with microbial DNA, as is typical of ...

科研

生物学

Quantification of &gamma;H2AX Foci in Response to Ionising Radiation

Quantification of &#947;H2AX Foci in Response to Ionising Radiation

电离辐射的反应定量的γH2AX灶

DNA double-strand breaks (DSBs), which are induced by either endogenous metabolic processes or by exogenous sources, are one of the most critical DNA ...

Imaging Cell Shape Change in Living <em>Drosophila</em> Embryos

Imaging Cell Shape Change in Living Drosophila Embryos

影像生活细胞的形状变化<em>果蝇</em>胚胎

The developing Drosophila melanogaster embryo undergoes a number of cell shape changes that are highly amenable to live confocal imaging.  Cell shape ...

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

表型分析和分离小鼠造血干细胞和传承的承诺祖

The bone marrow is the principal site where HSCs and more mature blood cells lineage progenitors reside and differentiate in an adult organism. HSCs ...

Live Imaging of Apoptotic Cell Clearance during <em>Drosophila </em>Embryogenesis

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

凋亡细胞清除过程中实时成像<em&gt;果蝇</em&gt;胚胎发育

The  proper elimination of unwanted or aberrant cells through apoptosis and  subsequent phagocytosis (apoptotic cell clearance) is crucial for normal  ...

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

隔离原住民土壤微生物潜力打破生物降解塑料地膜应用于农业

Fungi native to agricultural soils that colonized commercially available biodegradable mulch (BDM) films were isolated and assessed for potential to ...

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases <em>In Vivo</em>

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

基于荧光引物延伸法确定转录起点和核糖核酸酶的切割位点<em&gt;在体内</em

Fluorescence based primer extension (FPE) is a molecular method to determine transcriptional starting points or processing sites of RNA molecules. This is ...

Measuring Glutathione-induced Feeding Response in Hydra

测量水润谷胱甘肽诱导饲养响应

Hydra is among the most primitive organisms possessing a nervous system and chemosensation for detecting reduced glutathione (GSH) for capturing the prey. ...

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

缺氧条件下培养细胞内源性核蛋白的共免疫沉淀测定

Low oxygen levels (hypoxia) trigger a variety of adaptive responses with the Hypoxia-inducible factor 1 (HIF-1) complex acting as a master regulator. ...

Light Spot-Based Assay for Analysis of <em>Drosophila</em> Larval Phototaxis

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

光斑分析分析<em>果蝇</em>拉瓦尔光轴

The larvae of Drosophila melanogaster show obvious light-avoiding behavior during the foraging stage. Drosophila larval phototaxis can be used as a model ...