Name: video bioinformatics analysis of human embryonic stem cell colony growth
Uploaded: 2010-05-20T17:00:00
Description: Watch this Scientific Journal Video about Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth at JoVE.com

<p class="jove_content">这种方法的发展成为可能由加州烟草相关疾病的研究计划，加州再生医学研究所，美国国家科学基金会IGERT授予视频生物信息学（＃0903667）在加州大学河滨分校（ <a href="http://www.cris.ucr.edu/IGERT/index.php">资金</a> http://www.cris.ucr <a href="http://www.cris.ucr.edu/IGERT/index.php">.edu / IGERT / index.php文件</a> ）。肖恩Forteno萨布丽娜林是从论文奖学金研究生部的支持下，由美国国立卫生研究院的MARC奖学金支持，并Shruthi萨蒂什南比亚是由研究生部奖学金支持。我们感谢为她准备的数字帮助安娜Trtchounian。我们也感谢山姆Alworth和斯内德Jastromb教我们如何使用CL - QUANT软件。 </p>

<p class="jove_title">第1部分：实验步骤</p><p class="jove_content">视频数据包含了丰富的信息。然而，这些信息往往是难以提取和手工完成人类和人员时间可能需要许多小时才能完成。人类的人工分析也受到解释和错误的变异。视频生物信息学涉及计算机软件的使用矿从视频图像中的特定数据。这种分析方法是快速，并能消除发生的错误的分析是通过人类手动。本文的目的是表现出人类胚胎干细胞菌落生长，使用视频生物?...

<p class="jove_content">迅速从视频图像中提取数据，视频生物信息学工具是强大的。我们量化人类胚胎干细胞菌落生长的协议演示视频生物信息学的应用到生物问题。这种方法是定量的和有趣的功能，从个别的人类胚胎干细胞的殖民地揭示数据。视频生物信息学的配方，可以发展到其他细胞的过程进行监控，如增殖，迁移，凋亡，细胞附着到基板，到邻近的细胞和细胞附着。配方的准确性进行了验证使用Photoshop使用CL -...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>mTeSR1 Human Embryonic Stem Cell Maintenance Medium</td>
<td>&nbsp;</td>
<td>Stem Cell Technologies</td>
<td>05850</td>
<td>or any suitable medium for hESC culture.</td>
</tr>
<tr>
<td>BD Matrigel</td>
<td>&nbsp;</td>
<td>BD Bioscience</td>
<td>356234</td>
<td>or other suitable substrate.</td>
</tr>
<tr>
<td>DMEM/F12 Basal Medium</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>11330-032</td>
<td>&nbsp;</td>
<tr>
<td>Phosphate Buffered Saline without Ca<sup>2+</sup> and Mg<sup>2+</sup></td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Accutase Enzyme Cell Detachment Medium</td>
<td>&nbsp;</td>
<td>eBioscience</td>
<td>00-4555-56</td>
<td>or other suitable detachment enzyme.</td>
</tr>
<tr>
<td>3mm Glass beads</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>11-312A</td>
<td>optional.</td>
</tr>
<tr>
<td>12-well Tissue Culture Plates</td>
<td>&nbsp;</td>
<td>BD Falcon</td>
<td>353043</td>
<td>or any other plate format.</td>
</tr>
<tr>
<td>Nikon BioStation CT/IM</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>or other incubator/microscope suitable for collecting video data.</td>
</tr>
<tr>
<td>CL-Quant software (Nikon) and/or Photoshop (Adobe).</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>
	

video bioinformatics analysis of human embryonic stem cell colony growth

<p class="jove_content">由于视频数据是复杂的，包括许多图像，视频素材的挖掘信息是很难做到的，没有计算机软件的援助。视频生物信息学是一个强大的的，从视频图像中提取的时空数据，使用计算机软件来进行约会的挖掘和分析的定量方法。在这篇文章中，我们引入了视频生物信息学方法，通过分析时间推移视频配备了视频成像相机在尼康BioStation的CT孵化器收集量化的人类胚胎干细胞（胚胎干细胞）的生长。在我们的实验中，连接到基质胶的人类胚胎干细胞菌落，48小时在BioStation CT拍摄。要确定这些殖民地的增长速度，配方使用CL - QUANT软件，该软件使用户能够从视频图像的数据中提取各类开发。为了准确地评价菌落生长，创建三个配方。第一分段成殖民地和背景，第二次提高了图像准确定义整个视频序列的殖民地，第三的图像测量的像素数，在随着时间的推移殖民地。这三个配方在BioStation CT收集的数据来分析个别的人类胚胎干细胞菌落生长率超过48小时的视频序列中运行。为了验证CL - QUANT食谱的真实性，同样的数据进行了分析手动使用Adobe Photoshop软件。当所获得的数据使用CL - QUANT食谱和Photoshop进行了比较，结果几乎相同，表明CL - QUANT食谱是真实的。这里所描述的方法可以适用于任何视频数据来衡量人类胚胎干细胞或其他细胞生长在殖民地的生长率。此外，其他视频生物信息学的配方可以如其他细胞迁移，细胞凋亡和细胞粘附过程的未来发展。</p>

Watch this Scientific Journal Video about Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth at JoVE.com

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth (Video) | JoVE

<p class="jove_content">视频生物信息学的自动化处理，分析，理解，并从微观的影片中提取的生物时空数据的数据挖掘。本文的目的是为了演示使用视频生物信息学方法测量人类胚胎干细胞集落增长的方法。</p>

视频人类胚胎干细胞克隆生长的生物信息学分析

Because video data are complex and are comprised of many images, mining information from video material is difficult to do without the aid of computer ...

Research

JoVE Journal

Biology

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin. (Video) | JoVE

人类胚胎干细胞传代的色调线与胰蛋白酶

In this video we demonstrate how our lab routinely passages HuES human embryonic stem cell lines with trypsin.  Human embryonic stem cells are artifacts ...

科研

生物学

Propagation of Human Embryonic Stem (ES) Cells

Propagation of Human Embryonic Stem ES Cells (Video) | JoVE

人类胚胎干细胞（ES细胞）的繁殖

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells (Video) | JoVE

从小鼠胚胎干细胞的造血干细胞的推导

A stem cell is defined as a cell with the capacity to both self-renew and generate multiple differentiated progeny.  Embryonic stem cells (ESC) are ...

Derivation of Human Embryonic Stem Cells by Immunosurgery

Derivation of Human Embryonic Stem Cells by Immunosurgery (Video) | JoVE

人类胚胎干细胞的推导Immunosurgery

The ability of human embryonic stem cells to self-renew and differentiate into all cell types of 
the body suggests that they hold great promise for both ...

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Chromatin Immunoprecipitation from Human Embryonic Stem Cells (Video) | JoVE

从人类胚胎干细胞的染色质免疫沉淀

The functional and structural complexity of the myriad of cells in metazoan organisms arises from a small number of stem cells. Stem cells are ...

Culture and Maintenance of Human Embryonic Stem Cells  

Culture and Maintenance of Human Embryonic Stem Cells (Video) | JoVE

文化和维护人类胚胎干细胞

Human embryonic stem (hES) cells must be monitored and cared for in order to maintain healthy, undifferentiated cultures. At minimum, the cultures must be ...

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells (Video) | JoVE

成人内皮细胞集落形成祖细胞的分离和大型规模扩张

This paper introduces a novel recovery strategy for endothelial colony forming progenitor cells (ECFCs) from heparinized but otherwise unmanipulated adult ...

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs (Video) | JoVE

隔离和动物血清自由膨胀的人脐血源性间质基质细胞（MSCs），内皮细胞集落形成祖细胞（ECFCs）

The umbilical cord is a rich source for progenitor cells with high proliferative potential including mesenchymal stromal cells (also termed mesenchymal ...

Freezing and Thawing Human Embryonic Stem Cells

Freezing and Thawing Human Embryonic Stem Cells (Video) | JoVE

人类胚胎干细胞冷冻和解冻

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells ...

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors (Video) | JoVE

胚胎干细胞分化成少突胶质前体

Oligodendrocytes are the myelinating cells of the central nervous system. For regenerative cell therapy in demyelinating diseases, there is significant ...