High Pressure Freezing and Cryo Sectioning of Thicker Cells
6:43
Examination of the Cells Using the Cryo Light Microscope
7:49
Collecting Tilt Series Using the Cryo TEM
9:08
Tomogram Reconstruction and Database
10:24
Tomogram Segmentation
11:22
Representative Movies
12:59
Conclusion
10:51
Movies and Animation
Wir zeigen hier, wie Elektronen cryotomography (ECT) zu verwenden, um auf die Ultrastruktur von Bakterienzellen in nahezu native Staaten zu studieren, "makromolekularen" (~ 4 nm) Auflösung.