Name: recording behavioral responses to reflection in crayfish
Uploaded: 2010-05-14T17:00:00
Description: Watch this Scientific Journal Video about Recording Behavioral Responses to Reflection in Crayfish at JoVE.com

NSERC研究的支持。

1。社会化<ol><li>扑灭现有的社会行列，每个小龙虾是采用7 - 14天，在一个单独的塑料容器（长30厘米× 17.5厘米（宽）x 13厘米深），过滤，汽水和一个小塑料花盆作为填充栖身之所。如果使用透明的容器，一个分频器（如纸巾）被放置在容器之间，以确保小龙虾是视觉隔离。 </li><li>孤立地停留在一个额外的3 - 14天的“隔离”组中的小龙虾。水是改变和小龙虾是美联储每周3次。 </li><li>要建立社会...

<ol>
	<li>Bruski, C. A., Dunham, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+importance+of+vision+in+agonistic+communication+of+the+crayfish+Orconectes+rusticus.+I:+an+analysis+of+bout+dynamics.">The importance of vision in agonistic communication of the crayfish Orconectes rusticus. I: an analysis of bout dynamics.</a> Behav. 103, 83-107 (1987).</li><li>Delgado-Morales, G., Hernandez-Falcon, J., Ramon, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Agnostic+behavior+in+crayfish:the+importance+of+sensory+inputs.">Agnostic behavior in crayfish:the importance of sensory inputs.</a> Crustaceana. 77 (1), 1-24 (2004).</li><li>Guiasu, R. C., Dunham, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inter-form+agonistic+contests+in+male+crayfishes,+Cambarus+robustus+(Decapoda,+Cambaridae).">Inter-form agonistic contests in male crayfishes, Cambarus robustus (Decapoda, Cambaridae).</a> Invert. Biol.. 117 (2), 144-154 (1998).</li><li>May, H. Y., Mercier, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responses+of+crayfish+to+a+reflective+environment+depend+on+dominance+status.">Responses of crayfish to a reflective environment depend on dominance status.</a> Can. J. Zool. 84 (8), 1104-1111 (2006).</li><li>May, H. Y., Mercier, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Joffre+Duration+of+socialization+influences+responses+to+a+mirror:+responses+of+dominant+and+subordinate+crayfish+diverge+with+time+of+pairing.">Joffre Duration of socialization influences responses to a mirror: responses of dominant and subordinate crayfish diverge with time of pairing.</a> J. Exp. Biol. 210, 4428-4436 (2007).</li><li>May, H. Y., Luebbert, J., Mercier, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responses+of+Crayfish+to+a+Visual+Stimulus+Provided+by+a+Conspecific.">Responses of Crayfish to a Visual Stimulus Provided by a Conspecific.</a> , (2008).</li></ol>

占主导地位的小龙虾花更多的时间在一个反射的环境观察表明他们认识到作为一个同种反射的图像，和足够的视觉线索引出方法的行为。使用活的小龙虾，而不是一个反映图​​像的类似的研究结果支持这样的解释。从属和孤立的小龙虾没有表现出这种反射环境的偏好，也不做下属的小龙虾似乎更愿意在一个立方体的一个活的小龙虾。因此，视觉线索的反应取决于主导地位排名。这是目前尚不清?...

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<td>Single chamber test tank</td>
<td>&nbsp;</td>
<td>Brock University Machine Shop</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>End-chamber test tank</td>
<td>&nbsp;</td>
<td>Brock University Machine Shop</td>
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<td>Isolation container</td>
<td>&nbsp;</td>
<td>Rubbermaid</td>
<td>&nbsp;</td>
<td>30 cm x 17.5 cm x 13 cm holes in lid to allow air and light to pass through when lid is on</td>
</tr>
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<td>Fighting chamber</td>
<td>&nbsp;</td>
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<td>48 cm x 25 cm x 13 cm</td>
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<td>Social housing chamber</td>
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<td>58 cm x 30 cm x 35 cm</td>
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<td>small plastic flower pot</td>
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<td>to serve as shelter</td>
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<td>Coolpix 4500 camera</td>
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<td>Nikon</td>
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<td>T120 video cassette recorder</td>
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<td>RCA</td>
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<td>Computer</td>
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<td>Windows Movie Maker or other video acquiring software</td>
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<td>webcam</td>
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<td>Logitech</td>
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<td>modelling clay</td>
<td>&nbsp;</td>
<td>Sculptee</td>
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<td>to make control cube</td>
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<td>red latex paint</td>
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<td>FolkArt</td>
<td>&nbsp;</td>
<td>to make control cube</td>
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<td>waterbase varnish</td>
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<td>FolkArt</td>
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<td>to make control cube</td>
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recording behavioral responses to reflection in crayfish

社会行为取决于从视觉，机械和嗅觉系统的感觉输入。一个重要的问题涉及每个指导行为感官方式的相对作用。已审查通过隔离的机械和化学感受刺激的视觉刺激的视觉输入的作用。在一些研究中的视觉输入（Bruski与邓纳姆，1987 年 。德尔加多莫拉莱斯等，2004）已删除眼罩或低光的强度，并已阐明剩余的感官模式的影响。另一种方法是研究在没有任何适当的机械和化学感受线索的视觉输入的影响。这种做法的目的是确定的视觉输入的独家作用。 我们使用两种方法，提供的视觉刺激，不提供化学和机械线索，以小龙虾。在第一种方法，小龙虾是录像的墙壁有一半是在镜子覆盖提供反射环境的水族馆，和另一半则是在非反射（哑光）塑料盖。这使小龙虾的反射和非反射环境之间的选择。反射环境提供视觉线索，在小龙虾的反映图像的形式，因为它整个坦克的一半移动，这些视觉线索是从非反射一半的坦克失踪。另一种方法是到录像带的小龙虾在每年年底由更小室分隔水族馆的行为，在一个小室在对面的小室提供视觉线索和惰性对象，提供一个非移动的视觉输入的小龙虾，非小龙虾的来源。 我们公布的结果表明，小龙虾的反射环境的反应取决于社会和主导地位排名。社会化的小龙虾花更多的时间在反射环境，并表现出比在非反射的环境中的某些行为，更经常有孤立的小龙虾没有表现出这种差异。小龙虾，在同性对安置开发的主导或从属的社会地位。反射的反应不同的主导和从属的小龙虾（2006年5月名士，名士，2007年）。反射面占主导地位的小龙虾花更多的时间，更频繁地进入反射的角落，在反射的角落里花费更多的时间比非反射面。下属的小龙虾走反向往往比非反射面的反射面。初步数据表明，在毗邻的一个小室的小龙虾（May 等人，2008年）所提供的视觉线索类似的效果。

Watch this Scientific Journal Video about Recording Behavioral Responses to Reflection in Crayfish at JoVE.com

Recording Behavioral Responses to Reflection in Crayfish

我们已经开发出两种方法，研究触觉和化学信号的情况下对行为视觉线索的影响。一种方法涉及在水族馆反光外墙摄录小龙虾的反应;其他检查提供一个透明的分区后面的一个活的小龙虾的视觉输入的影响。

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