Name: recording behavioral responses to reflection in crayfish
Uploaded: 2010-05-14T17:00:00
Description: Watch this Scientific Journal Video about Recording Behavioral Responses to Reflection in Crayfish at JoVE.com

NSERC अनुसंधान द्वारा समर्थित है.

1. समाजीकरण <ol><li> मौजूदा सामाजिक रैंकों को बुझाने के लिए, प्रत्येक क्रेफ़िश 7 के लिए रखे जाते है - एक अलग प्लास्टिक कंटेनर (30 सेमी लंबे x 17.5 सेमी चौड़ी गहरी x 13 सेमी) में 14 दिन, फ़िल्टर, वातित जल और जो एक के रूप में ?...

<ol>
	<li>Bruski, C. A., Dunham, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+importance+of+vision+in+agonistic+communication+of+the+crayfish+Orconectes+rusticus.+I:+an+analysis+of+bout+dynamics.">The importance of vision in agonistic communication of the crayfish Orconectes rusticus. I: an analysis of bout dynamics.</a> Behav. 103, 83-107 (1987).</li><li>Delgado-Morales, G., Hernandez-Falcon, J., Ramon, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Agnostic+behavior+in+crayfish:the+importance+of+sensory+inputs.">Agnostic behavior in crayfish:the importance of sensory inputs.</a> Crustaceana. 77 (1), 1-24 (2004).</li><li>Guiasu, R. C., Dunham, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inter-form+agonistic+contests+in+male+crayfishes,+Cambarus+robustus+(Decapoda,+Cambaridae).">Inter-form agonistic contests in male crayfishes, Cambarus robustus (Decapoda, Cambaridae).</a> Invert. Biol.. 117 (2), 144-154 (1998).</li><li>May, H. Y., Mercier, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responses+of+crayfish+to+a+reflective+environment+depend+on+dominance+status.">Responses of crayfish to a reflective environment depend on dominance status.</a> Can. J. Zool. 84 (8), 1104-1111 (2006).</li><li>May, H. Y., Mercier, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Joffre+Duration+of+socialization+influences+responses+to+a+mirror:+responses+of+dominant+and+subordinate+crayfish+diverge+with+time+of+pairing.">Joffre Duration of socialization influences responses to a mirror: responses of dominant and subordinate crayfish diverge with time of pairing.</a> J. Exp. Biol. 210, 4428-4436 (2007).</li><li>May, H. Y., Luebbert, J., Mercier, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responses+of+Crayfish+to+a+Visual+Stimulus+Provided+by+a+Conspecific.">Responses of Crayfish to a Visual Stimulus Provided by a Conspecific.</a> , (2008).</li></ol>

अवलोकन है कि प्रमुख क्रेफ़िश एक चिंतनशील वातावरण में अधिक समय बिताने से पता चलता है कि वे एक conspecific के रूप में परिलक्षित छवि पहचान है, और उस दृश्य cues दृष्टिकोण व्यवहार प्रकाश में लाना करने के लिए पर्याप्त ?...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Single chamber test tank</td>
<td>&nbsp;</td>
<td>Brock University Machine Shop</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>End-chamber test tank</td>
<td>&nbsp;</td>
<td>Brock University Machine Shop</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Isolation container</td>
<td>&nbsp;</td>
<td>Rubbermaid</td>
<td>&nbsp;</td>
<td>30 cm x 17.5 cm x 13 cm holes in lid to allow air and light to pass through when lid is on</td>
</tr>
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<td>Fighting chamber</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>48 cm x 25 cm x 13 cm</td>
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<td>Social housing chamber</td>
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<td>58 cm x 30 cm x 35 cm</td>
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<td>small plastic flower pot</td>
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<td>to serve as shelter</td>
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<td>Coolpix 4500 camera</td>
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<td>Nikon</td>
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<td>T120 video cassette recorder</td>
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<td>RCA</td>
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<td>Computer</td>
<td>&nbsp;</td>
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<td>Windows Movie Maker or other video acquiring software</td>
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<td>webcam</td>
<td>&nbsp;</td>
<td>Logitech</td>
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<td>modelling clay</td>
<td>&nbsp;</td>
<td>Sculptee</td>
<td>&nbsp;</td>
<td>to make control cube</td>
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<td>red latex paint</td>
<td>&nbsp;</td>
<td>FolkArt</td>
<td>&nbsp;</td>
<td>to make control cube</td>
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<td>waterbase varnish</td>
<td>&nbsp;</td>
<td>FolkArt</td>
<td>&nbsp;</td>
<td>to make control cube</td>
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recording behavioral responses to reflection in crayfish

Social behavior depends on sensory input from the visual, mechanical and olfactory systems. One important issue concerns the relative roles of each sensory modality in guiding behavior. The role of visual inputs has been examined by isolating visual stimuli from mechanical and chemosensory stimuli. In some studies (Bruski &amp; Dunham, 1987: Delgado-Morales et al., 2004) visual inputs have been removed with blindfolds or low light intensity, and effects of remaining sensory modalities have been elucidated. An alternative approach is to study the effects of visual inputs in the absence of any appropriate mechanical and chemosensory cues. This approach aims to identify the exclusive role of visual inputs. 
We have used two methods to provide visual stimuli to crayfish without providing chemical and mechanical cues. In one method, crayfish are videotaped in an aquarium where half of the walls are covered in mirrors to provide a reflective environment, and the other half are covered in a non-reflective (matte finish) plastic. This gives the crayfish a choice between reflective and non-reflective environments. The reflective environment provides visual cues in the form of reflected images of the crayfish as it moves throughout half of the tank; these visual cues are missing from the non-reflective half of the tank. An alternative method is to videotape the behavior of crayfish in an aquarium separated by a smaller chamber at each end, with a crayfish in one small chamber providing visual cues and an inert object in the opposite small chamber providing visual input from a non-moving, non-crayfish source. 
Our published results indicate that responses of crayfish to the reflective environment depend on socialization and dominance rank. Socialized crayfish spent more time in the reflective environment and exhibited certain behaviors more frequently there than in the non-reflective environment; isolated crayfish showed no such differences. Crayfish that were housed in same-sex pairs developed a social rank of either dominant or subordinate. Responses to reflection differed between dominant and subordinate crayfish (May &amp; Mercier, 2006; May &amp; Mercier, 2007). Dominant crayfish spent more time on the reflective side, entered reflective corners more frequently and spent more time in reflective corners compared to the non-reflective side. Subordinate crayfish walked in reverse more often on the reflective side than on the non-reflective side. Preliminary data suggest similar effects from visual cues provided by a crayfish in a small adjoining chamber (May et al., 2008).

Watch this Scientific Journal Video about Recording Behavioral Responses to Reflection in Crayfish at JoVE.com

Recording Behavioral Responses to Reflection in Crayfish

हम व्यवहार पर स्पर्श और रासायनिक संकेत के अभाव में दृश्य cues के प्रभाव के अध्ययन के लिए दो तरीके विकसित किया है. एक विधि में एक मछलीघर में चिंतनशील दीवारों वीडियोटैपिंग क्रेफ़िश की प्रतिक्रियाओं शामिल है, अन्य दृश्य एक पारदर्शी विभाजन के पीछे एक जीवित क्रेफ़िश द्वारा प्रदान की आदानों के प्रभाव की जाँच.

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Social behavior depends on sensory input from the visual, mechanical and olfactory systems.  One important issue concerns the relative roles of each ...

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