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Please note that all translations are automatically generated. Click here for the English version.
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01:18 min
March 17th, 2023
DOI :
10.3791/200012-v
Transcript
为了对线粒体受体细胞系进行线粒体耗竭,使用含有10%FBS和青霉素-链霉素的完整培养基在六孔板中接种100万个细胞。每天用最佳浓度的罗丹明6G处理细胞三到七天,具体取决于细胞系的类型。为了保持细胞存活,用每毫升尿苷50微克和每毫升丙酮酸100微克补充细胞培养基。
处理后,在与从供体细胞系分离的线粒体融合之前,除去罗丹明6G处理细胞的培养基,并加入不含罗丹明6G的完整细胞培养基。将细胞在 37 摄氏度下与 5% 二氧化碳孵育三到四个小时。最后,为了制备用于融合的罗丹明6G预处理细
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