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Please note that all translations are automatically generated. Click here for the English version.
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02:19 min
March 17th, 2023
DOI :
10.3791/200014-v
Transcript
对于透射软骨细胞的生成,使用通过罗丹明6G处理耗尽为线粒体的线粒体受体细胞,并与从线粒体供体细胞中分离的线粒体进行融合。通过在六孔板的完整培养基中接种少量罗丹明 6G 处理的细胞和分离的线粒体,确保受体细胞中的线粒体功能适当消除和供体细胞的细胞器纯化。培养一个月后检查孔中没有存活细胞残留。
为了进行融合,小心地将罗丹明6G处理的细胞添加到分离的线粒体沉淀中。然后以520g离心五分钟,使细胞与线粒体混合。加入 100 微升 50% 聚乙二醇,轻轻重悬沉淀 30 秒,然后让悬浮液原
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