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Please note that all translations are automatically generated. Click here for the English version.
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01:44 min
May 5th, 2023
DOI :
10.3791/200041-v
Transcript
要开始线粒体标记,请在DMEM中用10%胎牛血清或FBS制备20微摩尔的BrdU溶液。然后加入线粒体示踪染料,将终浓度调节至1.1微摩尔。15小时后,将10微升线粒体示踪染料溶液加入含有孔的HeLa细胞悬液中。
孵育细胞一小时。用于用BrdU标记新合成的DNA分子的条件允许在HeLa细胞的线粒体中检测BrdU标记的DNA。用抗BrdU抗体获得的信号是特异性的,仅在用BrdU处理的细胞中观察到。
无论BrdU治疗如何,在零行细胞的线粒体中均未检测到抗BrdU或抗DNA
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