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Please note that all translations are automatically generated. Click here for the English version.
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03:57 min
April 14th, 2023
DOI :
10.3791/200057-v
Transcript
首先对聚四氟乙烯或PTFE涂层载玻片进行灭菌,方法是将它们浸入70%乙醇中生物安全柜中10分钟。让载玻片在无菌的100毫米细胞培养皿中风干。将每张载玻片放入 1 个新的 100 毫米细胞培养皿中,并向每个矩形中加入 200 微升含血清的细胞培养基。
然后将手持式254纳米紫外灯放在100毫米细胞培养皿上,灯泡直接面向载玻片20分钟。在 37 摄氏度的水浴中解冻外段或 OS。然后在室温下以 2, 400G 旋转操作系统五分钟。
立即使用移液管吸出生物安全柜中的上清液,
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