Preparation of Bioactive Compound Encapsulated Chitosan Nanoparticles

To begin, prepare chitosin solution by dissolving 0.6 grams of chitosin flakes in 50 milliliters of 1%acetic acid. Agitate overnight at room temperature to get a homogenous emulsion. Add 0.5 grams of Tween 80 and stir at 60 degrees Celsius for two