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Please note that all translations are automatically generated. Click here for the English version.
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01:24 min
April 7th, 2023
DOI :
10.3791/200063-v
Transcript
首先,通过将0.6克壳肽片溶解在50毫升1%乙酸中来制备壳素溶液。在室温下搅拌过夜,得到均匀的乳液。加入 0.5 克吐温 80,在 60 摄氏度下以 1, 000 RPM 搅拌两小时,以获得均匀的溶液。
将溶液降至室温后,滴加或逐渐加入0.75克生物活性化合物香芹酚或麝香草酚,同时搅拌混合物20分钟。在室温下搅拌的同时,将50毫升三聚磷酸五钠滴加到混合物中。继续搅拌30分钟以获得均匀的乳液。
为了纯化,将乳液在10, 000 G下在4摄氏度下离心30分钟。倒出上清液
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