Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
301 Views
•
01:44 min
April 7th, 2023
DOI :
10.3791/200064-v
Transcript
取经过纳米颗粒处理的织物,将其切割成50毫米乘25毫米的色板。将新鲜纯度板中的微生物培养物接种到35摄氏度的胰蛋白酶大豆肉汤中过夜,并适度摇动。将过夜培养物稀释至每毫升1.5亿CFU,或相当于0.5麦克法兰标准品的浊度。
为了用四毫米的无菌环接种稀释的培养物,加载一个装满肉汤培养物的环,并通过制作五条长度为6厘米,彼此相距1厘米的平行条纹将其转移到Mueller-Hinton琼脂或MHA琼脂平板的表面。使用无菌刮刀在五条条纹上轻轻按压织物色板,使织物位于中心并接触所有五条条纹线
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved