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Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers

DOI :

10.3791/200065-v

•

June 2nd, 2023

June 2nd, 2023

•
105 Views
1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine

Tags

Construct Design
Transfecting
Cysteine engineered
Voltage gated Ca2 Channels
Murine Isolated Skeletal Muscle Fibers
Wild type
Fluorescently tagged CaV1 1 CDNA Construct
Plasmid Coating
Enhanced Green Fluorescent Protein
EGFP tagged Alpha Subunit
Rabbit Cav1 1
Cytomegalovirus Promoter
Transfection Efficiency
Muscle Fibers
Cystine Substitution
Fluorometry
Site directed Mutagenesis Kit
Cysteines Insertion
Membrane Extracellular Interface
S4
GFP Observation
Ionic Current Recording
Voltage Dependence
Conductance

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