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Please note that all translations are automatically generated. Click here for the English version.
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03:18 min
April 21st, 2023
DOI :
10.3791/200072-v
Transcript
首先将 500 μL 裂解缓冲液添加到先前获得的 A2780 细胞沉淀中,并使用最小开口直径为 2 毫米的切头轻轻混合。每毫升加入20微克新鲜制备的RNase,并通过温和的倒置混合。在 37 摄氏度下孵育 30 分钟。
在孵育结束时,加入蛋白酶K至每毫升100微克的终浓度,轻轻倒置10次。在 55 摄氏度下再次孵育一小时,每 10 分钟倒置试管。将500微升苯酚:氯仿:异戊醇试剂移入试管中,轻轻倒置试管约20次。
在室温下以9, 391 G离心15分钟。将上层水性粘
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