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01:29 min
May 26th, 2023
DOI :
10.3791/200097-v
Transcript
将Fura-2 AM等分试样溶解在Tyrode溶液中,以获得1微摩尔Fura-2 AM的最终浓度以添加到细胞中。吸出培养基并用含有Fura-2 AM的Tyrode溶液代替。在培养箱或基于光学的测量系统中在 37 摄氏度下孵育 15 分钟。取出含有Fura-2 AM的Tyrode溶液,并用新鲜的Tyrode溶液清洗孔两次。
最后,将Tyrode溶液中的细胞在37摄氏度下再孵育五分钟,以使Fura-2 AM脱酯。将板放在基于光学的测量系统中以进行收缩性测量。选择一个孔,选择孔内的一个区域以观察同步收缩和松弛,然后单击“开始”,并添加测量值。除了收缩痕迹外,屏幕上还会出现实时比例钙瞬变。
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