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Please note that all translations are automatically generated. Click here for the English version.
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02:32 min
April 28th, 2023
DOI :
10.3791/200099-v
Transcript
对于这项研究,使用在水稻幼苗中饲养的小棕色飞虱或SBPH。培养物准备好后,将生长的SBPH放入离心管中,并将它们置于冰浴中10至30分钟。然后将冷冻的SBPH放在体视显微镜下的载玻片上,腹部朝上,并调整其六条腿的焦点。
准备两个带有超细尖端的高精度镊子。使用一个镊子,按下昆虫的身体以将其保持在适当的位置,然后用另一把镊子小心地拉下一条腿。然后轻轻按压昆虫的胸部,让血淋巴流过伤口。
为了收集血淋巴,通过将一微升毛细管放入移液管球中来准备微量移液器。将毛细管靠近昆虫伤口
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