﻿parameter acquisition for dlp printing

Caracterização quantitativa das propriedades da biotinta para impressão DLP contínua

Tissue engineering1 is important in the field of organ repair. Due to the lack of organ donation, some diseases, such as liver failure and kidney failure, cannot be cured well, and many patients do not receive timely treatment2. Organoids with the required function of the organs may solve the problem caused by the lack of organ donation. The construction of organoids depends on the progress and development of bioprinting technology3.
Compared with extrusion-type bioprinting4 and inkjet-type bioprinting5, the printing speed and printing accuracy of the digital light processing (DLP) bioprinting method are higher6,7. The printing module of the extrusion-type method is line-by-line, while the printing module of the inkjet-type method is dot-by-dot, which is less efficient than the layer-by-layer printing module of DLP bioprinting. The modulated ultraviolet (UV) light exposure to a whole layer of material to cure a layer in DLP bioprinting and the feature size of the image determines the accuracy of DLP printing. This makes DLP technology very efficient8,9,10. Due to overcuring of the UV light, the precise relationship between the curing time and the printing size is important for high-accuracy DLP bioprinting. Furthermore, continuous DLP printing is a modification of DLP printing method that can greatly improve the printing efficiency11,12,13. For continuous DLP printing, precise printing conditions are the most important factors.
The relationship between the curing time and the printing size is called the Jacobs working curve, which is widely used in DLP printing14,15,16. The traditional method to obtain the relationship is to expose the material for a certain time and measure the curing thickness to obtain a data point about the exposure time and curing thickness. Repeating this operation at least five times and fitting the data points obtains the Jacobs working curve. However, this method has obvious disadvantages; it needs to consume a lot of material to achieve the curing, the results are highly dependent on the printing conditions, the bioinks used in DLP bioprinting are expensive and rare, and the formability of the bioinks is usually not good, which can lead to inaccurate measurements of curing thickness.
This article provides a new method to obtain the curing relationship according to the physical properties of the bioink. Using this theory can optimize continuous DLP printing. This method can be used to obtain the curing relationship more quickly and accurately; the continuous DLP curing can therefore be better determined.

Autor em destaque: Caracterização quantitativa de propriedades de biotinta fotossensível líquida para impressão baseada em processamento contínuo de luz digital  

Caracterização quantitativa das propriedades da biotinta fotossensível líquida para impressão baseada em processamento contínuo de luz

My research mainly involves DLP 3D bio printing and tissue engineering. We try to optimize and improve the DLP printing process and formula theory, so that the printed tissue engineering scaffold can better meet the actual clinic application needs. The printing conditions of the existing light curable materials rely on manual experiments and are obtained by feeding the data of multiple performing experiments.Such experiments waste material and reduced printing efficiency. I constructed a theoretical working curve and determined the DLP printing pyramids only based on the physical properties of the material. Dynamics both best concept is proposed to be applied in DLP printing to realize DLP format of complex dimensional structures of extremely soft materials.This method can improve the efficiency of determining the printing conditions of photo curable materials. This technology can provide technical support for the research and development of photo curable materials and the accurate printing of these materials.

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Parameter Acquisition for DLP Printing  

Aquisição de parâmetros para impressão DLP

Comece medindo o tempo limite da biotinta usando um reômetro com um elemento de controle de temperatura. Utilize uma fonte de luz de 365 nanômetros para expor a plataforma de teste do reômetro e defina a intensidade da luz para um determinado valor. No software do reômetro, vá para a opção de configurações de tempo e defina o reômetro para adquirir os dados de módulos de tempo para 300 pontos de dados com cada ponto de dados tomado a cada 0,3 segundos.Clique no botão Iniciar teste no software do reômetro para iniciar o teste. Simultaneamente, clique no botão Iniciar da fonte de luz. Para calcular o tempo limite, registre a contagem de tempo correspondente a partir do início da exposição quando os dados do módulo de armazenamento forem iguais aos dados do módulo de perda.Em seguida, construa o equipamento de teste de absorbância usando lâminas de vidro superiores e inferiores e a estrutura impressa em forma de anel. Aperte o anel entre as corrediças de vidro para que o círculo interno do anel forme a câmara. Coloque a câmara na área de ensaio do medidor de intensidade luminosa e ajuste a fonte de luz para expor a área da câmara.Quando a câmara de ensaio não estiver preenchida com material do equipamento de ensaio de absorbância, medir a intensidade luminosa incidente, II, através da leitura do visor do medidor de intensidade luminosa. Em seguida, encha a câmara de teste com 10 microlitros de biotinta. Expor a câmara de teste contendo biotinta à luz ultravioleta a 365 nanômetros.Obtenha a intensidade da luz, IIH, lendo o visor. Quando o valor não mudar mais no medidor de intensidade de luz, leia o visor para obter a intensidade da luz quando a biotinta estiver curada. O valor é a absorvância sólida, ISH.

Research

JoVE Journal

Bioengineering

﻿Parameter Acquisition for DLP Printing

Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

Precise printing fabrication of bioinks is a prerequisite for tissue engineering; the Jacobs working curve is the tool to determine the precise printing ...

Bioengenharia

Continuous DLP Printing Parameter Settings

Configurações de parâmetro de impressão DLP contínua