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Please note that all translations are automatically generated. Click here for the English version.
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04:22 min
May 12th, 2023
DOI :
10.3791/200116-v
Transcript
首先将含有冷冻小瓶的冷冻解离胚胎心脏细胞悬液放入37摄氏度的水浴中一到两分钟。将一毫升在37摄氏度下预热的完全培养基加入解冻的悬浮液中,并用移液管混合三次。然后将悬浮液转移到装有10毫米温完全培养基的15毫升锥形管中。
将整个细胞悬液通过 30 微米过滤器,并用一到两毫升温热的完全培养基冲洗过滤器。将流经的流量收集在同一锥形管中。接下来,将试管以 300 G 离心五分钟。
除去上清液后,用PBS洗涤沉淀并将细胞悬液转移到1.5毫升微管中。离心细胞悬液,并向沉淀的细胞
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