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Please note that all translations are automatically generated. Click here for the English version.
168 Views
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01:45 min
May 12th, 2023
DOI :
10.3791/200117-v
Transcript
首先,用70%乙醇和RNase去除溶液清洁工作场所和材料。新鲜制备裂解缓冲液、裂解稀释缓冲液、0.1 x 裂解缓冲液和洗涤缓冲液,并将缓冲液保持在冰上。预冷水平吊桶离心机至4摄氏度。
在摆动桶离心机中离心心脏祖细胞悬液。轻轻除去所有上清液,不要接触管底部。向细胞悬液中加入100微升冷冻的0.1x裂解缓冲液,并通过移液10次混合。
在冰上孵育五分钟后,加入一毫升冷冻洗涤缓冲液并混合。离心悬浮液后,除去上清液并用冷却的洗涤缓冲液再重复两次洗涤。准备稀释的细胞核缓冲液。<
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