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Please note that all translations are automatically generated. Click here for the English version.
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01:38 min
May 12th, 2023
DOI :
10.3791/200126-v
Transcript
首先在含有富集培养基的T25烧瓶中培养副结核分枝杆菌K-10菌株,在37摄氏度下生长两周。通过目视检查定期评估菌落生长。在摇动培养箱中的500毫升锥形瓶中培养200毫升富集培养物一周。
将细菌悬浮液置于 70 摄氏度下 5 至 10 分钟以使其灭活,然后在 3000G 下离心 10 分钟。在20毫升PBS中洗涤颗粒两次,然后离心悬浮液。将沉淀转移到无菌容器中并用液氮冷冻。
立即将冷冻沉淀转移到冻干室中。冻干后将其转移到生物清洁工作台上。使用不锈钢刮刀将粘附在容器内壁
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