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Please note that all translations are automatically generated. Click here for the English version.
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01:06 min
April 21st, 2023
DOI :
10.3791/200137-v
Transcript
首先,取消化的稀缺细胞的DNA并将其放在冰上。使用填充和生物素化限制性切合片段突出部所需的试剂制备预混液。将样品在 37 摄氏度下孵育 75 分钟,每 15 分钟倒置混合一次。
将样品在冰上冷却,并使用连接所需的试剂制备预混液来连接填充的DNA末端。最后,将样品在 16 摄氏度下孵育四到六个小时,然后在室温下孵育 30 分钟。每毫升蛋白酶K加入30微升10毫克以去交联染色质。
混合溶液,并在65摄氏度下孵育过夜。
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