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01:36 min
April 21st, 2023
DOI :
10.3791/200148-v
Transcript
病毒感染三周后,麻醉并用头板固定在立体定位框架上的小鼠。将凝胶泡沫浸泡在生理盐水中以止血。使用微型钻头创建一个 3 x 2 毫米的椭圆形,中心位于 lambda 后方 0.5 毫米处。
将椭圆形的边界变薄,直到它破裂,然后在椭圆形记录窗口之前变薄头骨,以关闭 SC 的盖玻片。使用细镊子取下骨瓣。切开横窦后方的硬脑膜,取出硬脑膜。用凝胶泡沫和棉签擦干头骨和记录窗口。
然后将一滴有机硅粘合剂沉积到记录窗口中。用吸盘负压握住吸盘,用电动显微机械手移动吸盘,将吸盘放下,放入
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