To begin, open the software invivo six, and click on browse file from this device. Select the scan to be analyzed and click open. Go to the 3D analysis module and click on the save information floppy disk icon.
Then select load a configuration and browse for the configuration file. Click on the reorientation icon. In the window that opens, select the by picking landmarks option.
This allows the user to orientate all the scans, in the same way. For this protocol nasion is selected as the origin landmark. Then three point definition with landmarks, right orbitally right porion left, porion, and define anterior, posterior axis, midsagittal plane with landmarks, nasion and basion are selected.
Adjust the brightness and contrast, to reduce the CBCT scam image noise from the menu on the left side of the screen. Zoom in and out by holding down the control key and left clicking simultaneously and sliding across the screen. Move the image in a bodily manner by holding down the shift key and left clicking simultaneously and sliding across the screen.
Enable clipping from the settings menu to create sectional views in all planes of space. To add new landmarks in the settings menu, click on landmarks, to reveal a list of available landmark options and then select the landmark of choice. To set a default view for landmarks, select tracing task.
Click on edit, choose landmark. Set the view as desired, and click on use current view settings. Repeat these steps to change the default view for any additional landmark.
To annotate 3D anatomical landmarks, select create tracing from the top of the menu, and click start. Start annotating the 3D landmarks by left clicking directly on the 3D volume at the location where the landmark should be placed, based on its definition. Adjust the location of the landmark using the section views on the right of the screen.
In case they cannot be seen from the layout selection menu, select slice locator. To confirm the placement of a landmark, click stop and select the desired view to visualize the landmark. Then proceed to place the remaining landmarks.
To change the position of the landmark using the 3D volume, stop the analysis by clicking on stop at the bottom of the tracing tasks menu. Click on the landmark point to be moved, and drag it to the new desired position. Re-annotate a landmark by double clicking on the ticked square next to the landmark and then answering yes to the follow-up question.
