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Please note that all translations are automatically generated. Click here for the English version.
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02:29 min
April 7th, 2023
DOI :
10.3791/200157-v
Transcript
固定安乐死小鼠的去核眼球后,将其转移到载玻片上并将其置于解剖显微镜下。使用镊子夹住角膜边缘以防止眼球运动。用解剖剪刀,通过角膜巩膜巩膜边缘在角膜周围切开约一毫米的深度,然后取下晶状体和玻璃体。
将视盘保持在中心,沿四个象限径向切割视网膜,达到视网膜半径的大约 2/3。用齿镊钳夹住巩膜边缘之一。用无齿镊子握住巩膜边缘,然后从带齿的镊子向巩膜底部移动。
小心地剥离视网膜。使用移液管用200微升PBS润湿视网膜。使用吸水纸去除多余的PBS,并在需要时用小毛刷压平视网膜。<
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