Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
379 Views
•
02:36 min
June 2nd, 2023
DOI :
10.3791/200167-v
Transcript
通过0.22微米的过滤膜过滤从Sipunculus nudus肠中提取的粗蛋白。将此样品储存在四摄氏度下以备后用。通过将精氨酸琼脂糖基质培养基装入5毫升空色谱柱中,将精氨酸琼脂糖基质亲和色谱柱装入精氨酸琼脂糖基质亲和色谱柱。
类似地,通过将赖氨酸琼脂糖基质介质加载到空色谱柱中来加载赖氨酸琼脂糖基质亲和色谱柱。用双蒸水平衡亲和色谱柱,然后用盐酸三缓冲液平衡。将过滤后的蛋白质样品加载到预平衡柱上。
用五体积的0.02摩尔三盐酸盐缓冲液洗涤色谱柱,然后依次用0.15-0.
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved