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Please note that all translations are automatically generated. Click here for the English version.
250 Views
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02:00 min
June 2nd, 2023
DOI :
10.3791/200168-v
Transcript
通过将25毫克纤维蛋白原与1.25毫升生理盐水混合开始纤维蛋白板制备。接下来,通过将100单位凝血酶与1.05毫升生理盐水彻底混合来制备凝血酶溶液。然后,通过将0.5克琼脂糖加入22.5毫升0.02摩尔Tris-盐酸盐酸缓冲液中来制备琼脂糖溶液。
将琼脂糖溶液加热至100摄氏度,直至完全溶解。将琼脂糖溶液冷却至约50摄氏度,然后再向其中添加纤维蛋白原溶液。立即加入凝血酶溶液,快速混合,然后将混合物倒入60毫米培养皿中。
要加载样品,请使用无菌布尔人将三毫米孔打入纤维
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