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Please note that all translations are automatically generated. Click here for the English version.
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01:51 min
May 19th, 2023
DOI :
10.3791/200188-v
Transcript
要执行单细胞剪切测定方案,请使用预组装的剪切设备设置,该装置设置由放置在包含感兴趣细胞的培养皿上的安装微流体流动室组成。将整个装置放在具有足够高的物镜和显示监视器的倒置显微镜上。聚焦显微镜物镜,确保足够的对比度和清晰的细胞边缘。
移动显微镜载物台,以确保细胞在显示器上清晰可见并且是实时图像。在安装的流动室和培养皿的成像或流路中选择一个或多个不同的细胞。为了保持连续均匀的粘性介质流,请选择相似的输注和退出速率。
确保雷诺数或RE小于100的流体的适当层流。单击剪切泵上
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